Ceratocystis cacaofunesta genome analysis reveals a large expansion of extracellular phosphatidylinositol-specific phospholipase-C genes (PI-PLC)

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Lopez Molano, Eddy Patricia ^[1] ; Cabrera, Odalys Garcia ^[1] ; Jose, Juliana ^[1] ; do Nascimento, Leandro Costa ^[2] ; Carazzolle, Marcelo Falsarella ^{[1, 2]} ; Pereira Lima Teixeira, Paulo Jose ^{[3, 1]} ; Correa Alvarez, Javier ^[4] ; Tiburcio, Ricardo Augusto ^[1] ; Tokimatu Filho, Paulo Massanari ^[1] ; Alvares de Lima, Gustavo Machado ^[5] ; Carvalho Guido, Rafael Victorio ^[5] ; Ribeiro Correa, Thamy Livia ^[1] ; Paes Leme, Adriana Franco ^[6] ; Mieczkowski, Piotr ^[7] ; Guimaraes Pereira, Goncalo Amarante ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Genom & Express Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Ctr Nacl Processamento Alto Desempenho, Campinas, SP - Brazil

^[3] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 - USA

^[4] Univ EAFIT, Escuela Ciencias, Dept Ciencias Biol, Medellin - Colombia

^[5] Univ Sao Paulo, Inst Fis Sao Carlos, Ctr Biotecnol Mol Estrutural, Sao Paulo - Brazil

^[6] Brazilian Biosci Natl Lab LNBio CNPEM, Campinas, SP - Brazil

^[7] Univ N Carolina, High Throughput Sequencing Facil, Chapel Hill, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 19, JAN 17 2018.
• Citações Web of Science: 3

Resumo

Background: The Ceratocystis genus harbors a large number of phytopathogenic fungi that cause xylem parenchyma degradation and vascular destruction on a broad range of economically important plants. Ceratocystis cacaofunesta is a necrotrophic fungus responsible for lethal wilt disease in cacao. The aim of this work is to analyze the genome of C. cacaofunesta through a comparative approach with genomes of other Sordariomycetes in order to better understand the molecular basis of pathogenicity in the Ceratocystis genus. Results: We present an analysis of the C. cacaofunesta genome focusing on secreted proteins that might constitute pathogenicity factors. Comparative genome analyses among five Ceratocystidaceae species and 23 other Sordariomycetes fungi showed a strong reduction in gene content of the Ceratocystis genus. However, some gene families displayed a remarkable expansion, in particular, the Phosphatidylinositol specific phospholipases-C (PI-PLC) family. Also, evolutionary rate calculations suggest that the evolution process of this family was guided by positive selection. Interestingly, among the 82 PI-PLCs genes identified in the C. cacaofunesta genome, 70 genes encoding extracellular PI-PLCs are grouped in eight small scaffolds surrounded by transposon fragments and scars that could be involved in the rapid evolution of the PI-PLC family. Experimental secretome using LC-MS/MS validated 24% (86 proteins) of the total predicted secretome (342 proteins), including four PI-PLCs and other important pathogenicity factors. Conclusion: Analysis of the Ceratocystis cacaofunesta genome provides evidence that PI-PLCs may play a role in pathogenicity. Subsequent functional studies will be aimed at evaluating this hypothesis. The observed genetic arsenals, together with the analysis of the PI-PLC family shown in this work, reveal significant differences in the Ceratocystis genome compared to the classical vascular fungi, Verticillium and Fusarium. Altogether, our analyses provide new insights into the evolution and the molecular basis of plant pathogenicity. (AU)

• Processo FAPESP: 09/50119-9 - Estudo integrado e comparativo de três doenças fúngicas do cacau: vassoura-de-bruxa, monilíase e mal do facão, visando à compreensão de mecanismos de patogenicidade para o desenvolvimento de estratégias de controle
• Beneficiário: Gonçalo Amarante Guimarães Pereira
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/18467-7 - Caracterização do perfil hormonal da doença vassoura-de-bruxa. significado dos hormônios produzidos por M. perniciosa para o desenvolvimento da doença
• Beneficiário: Odalys García Cabrera
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
• Beneficiário: Munir Salomao Skaf
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs