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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-33 Receptor (ST2) Deficiency Improves the Outcome of Staphylococcus aureus-lnduced Septic Arthritis

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Staurengo-Ferrari, Larissa [1] ; Trevelin, Silvia C. [2, 3] ; Fattori, Victor [1] ; Nascimento, Daniele C. [2] ; de Lima, Kalil A. [2] ; Pelayo, Jacinta S. [4] ; Figueiredo, Florencio [5] ; Casagrande, Rubia [6] ; Fukada, Sandra Y. [7] ; Teixeira, Mauro M. [8] ; Cunha, Thiago M. [2] ; Liew, Foo Y. [9] ; Oliveira, Rene D. [10] ; Louzada-Junior, Paulo [10] ; Cunha, Fernando Q. [2] ; Alves-Filho, Jose C. [2] ; Verri, Waldiceu A. [1]
Número total de Autores: 17
Afiliação do(s) autor(es):
[1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Patol, Londrina - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto - Brazil
[3] Kings Coll London, British Heart Fdn Ctr, Cardiovasc Div, London - England
[4] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Microbiol, Londrina - Brazil
[5] Univ Brasilia, Fac Med, Lab Pathol, Brasilia, DF - Brazil
[6] Univ Estadual Londrina, Hlth Sci Ctr, Dept Pharmaceut Sci, Londrina - Brazil
[7] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ribeirao Preto - Brazil
[8] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Lab Imunofarmacol, Belo Horizonte, MG - Brazil
[9] Univ Glasgow, Div Immunol Infect & Inflammat, Glasgow, Lanark - Scotland
[10] Univ Sao Paulo, Sch Med Ribeirao Preto, Div Clin Immunol, Ribeirao Preto - Brazil
Número total de Afiliações: 10
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN IMMUNOLOGY; v. 9, MAY 16 2018.
Citações Web of Science: 2
Resumo

The ST2 receptor is a member of the Toll/IL-1 R superfamily and interleukin-33 (IL-33) is its agonist Recently, it has been demonstrated that IL-33/ST2 axis plays key roles in inflammation and immune mediated diseases Here, we investigated the effect of ST2 deficiency in Staphylococcus auretus-induced septic arthritis physiopathology Synovial fluid samples from septic arthritis and osteoarthritis individuals were assessed regarding IL-33 and soluble (s) ST2 levels The IL-33 levels in samples from synovial fluid were significantly increased, whereas no sST2 levels were detected in patients with septic arthritis when compared with osteoarthritis individuals The intra-articular injection of 1 x 10(7) colony-forming unity/10 mu l of S. aureus American Type Culture Collection 6538 in wild-type (WT) mice induced IL-33 and sST2 production with a profile resembling the observation in the synovial fluid of septic arthritis patients Data using WT, and ST2 deficient ((-/-)) and interferon-gamma (IFN-gamma)(-/-) mice showed that ST2 deficiency shifts the immune balance toward a type 1 immune response that contributes to eliminating the infection due to enhanced microbicide effect via NO production by neutrophils and macrophages In fact, the treatment of ST2(-/-) bone marrow-derived macrophage cells with anti-IFN-gamma abrogates the beneficial phenotype in the absence of ST2, which confirms that ST2 deficiency leads to IFN-gamma expression and boosts the bacterial killing activity of macrophages against S. aureus In agreement, WT cells achieved similar immune response to ST2 deficiency by IFN-gamma treatment The present results unveil a previously unrecognized beneficial effect of ST2 deficiency in S. aureus-induced septic arthritis. (AU)

Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
Beneficiário:Fernando de Queiroz Cunha
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 11/19670-0 - Mecanismos envolvidos na fisiopatologia da artrite reumatóide, dor e sepse
Beneficiário:Fernando de Queiroz Cunha
Linha de fomento: Auxílio à Pesquisa - Temático