| Full text | |
| Author(s): Show less - |
Staurengo-Ferrari, Larissa
[1]
;
Trevelin, Silvia C.
[2, 3]
;
Fattori, Victor
[1]
;
Nascimento, Daniele C.
[2]
;
de Lima, Kalil A.
[2]
;
Pelayo, Jacinta S.
[4]
;
Figueiredo, Florencio
[5]
;
Casagrande, Rubia
[6]
;
Fukada, Sandra Y.
[7]
;
Teixeira, Mauro M.
[8]
;
Cunha, Thiago M.
[2]
;
Liew, Foo Y.
[9]
;
Oliveira, Rene D.
[10]
;
Louzada-Junior, Paulo
[10]
;
Cunha, Fernando Q.
[2]
;
Alves-Filho, Jose C.
[2]
;
Verri, Waldiceu A.
[1]
Total Authors: 17
|
| Affiliation: | [1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Patol, Londrina - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto - Brazil
[3] Kings Coll London, British Heart Fdn Ctr, Cardiovasc Div, London - England
[4] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Microbiol, Londrina - Brazil
[5] Univ Brasilia, Fac Med, Lab Pathol, Brasilia, DF - Brazil
[6] Univ Estadual Londrina, Hlth Sci Ctr, Dept Pharmaceut Sci, Londrina - Brazil
[7] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ribeirao Preto - Brazil
[8] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Lab Imunofarmacol, Belo Horizonte, MG - Brazil
[9] Univ Glasgow, Div Immunol Infect & Inflammat, Glasgow, Lanark - Scotland
[10] Univ Sao Paulo, Sch Med Ribeirao Preto, Div Clin Immunol, Ribeirao Preto - Brazil
Total Affiliations: 10
|
| Document type: | Journal article |
| Source: | FRONTIERS IN IMMUNOLOGY; v. 9, MAY 16 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
The ST2 receptor is a member of the Toll/IL-1 R superfamily and interleukin-33 (IL-33) is its agonist Recently, it has been demonstrated that IL-33/ST2 axis plays key roles in inflammation and immune mediated diseases Here, we investigated the effect of ST2 deficiency in Staphylococcus auretus-induced septic arthritis physiopathology Synovial fluid samples from septic arthritis and osteoarthritis individuals were assessed regarding IL-33 and soluble (s) ST2 levels The IL-33 levels in samples from synovial fluid were significantly increased, whereas no sST2 levels were detected in patients with septic arthritis when compared with osteoarthritis individuals The intra-articular injection of 1 x 10(7) colony-forming unity/10 mu l of S. aureus American Type Culture Collection 6538 in wild-type (WT) mice induced IL-33 and sST2 production with a profile resembling the observation in the synovial fluid of septic arthritis patients Data using WT, and ST2 deficient ((-/-)) and interferon-gamma (IFN-gamma)(-/-) mice showed that ST2 deficiency shifts the immune balance toward a type 1 immune response that contributes to eliminating the infection due to enhanced microbicide effect via NO production by neutrophils and macrophages In fact, the treatment of ST2(-/-) bone marrow-derived macrophage cells with anti-IFN-gamma abrogates the beneficial phenotype in the absence of ST2, which confirms that ST2 deficiency leads to IFN-gamma expression and boosts the bacterial killing activity of macrophages against S. aureus In agreement, WT cells achieved similar immune response to ST2 deficiency by IFN-gamma treatment The present results unveil a previously unrecognized beneficial effect of ST2 deficiency in S. aureus-induced septic arthritis. (AU) | |
| FAPESP's process: | 13/08216-2 - CRID - Center for Research in Inflammatory Diseases |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Projects - Thematic Grants |