Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats

Autor(es)

Francescato, Heloisa D. C. ^[1] ; Almeida, Lucas F. ^[1] ; Reis, Natany G. ^[1] ; Faleiros, Camila M. ^[1] ; Papoti, Marcelo ^[2] ; Costa, Roberto S. ^[3] ; Coimbra, Terezila M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Phys Educ & Sports Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pathol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Kidney & Blood Pressure Research; v. 43, n. 2, p. 582-593, 2018.
• Citações Web of Science: 2

Resumo

Background/Aims: Physical training has beneficial effects on endothelial function and can influence the regeneration of the endothelial cell. We investigated the effect of physical training on cisplatin (CP)-induced acute kidney injury and assessed the impact of training on endothelial structure and function, and on the inflammatory processes in rats. Methods: We injected male Wistar rats subjected to previous physical training in treadmill running (trained, TR) or not (sedentary, SED) with CP (5 mg/kg) (TR+CP and SED+CP groups, respectively). Five days after the injections, blood and urine samples were collected to evaluate renal function and kidneys were harvested for morphological, immunohistochemical, enzyme-linked immunosorbent assay, and analysis of nitric oxide (NO) levels. Results: Rats treated with CP showed increased levels of plasma creatinine and sodium and potassium fractional excretion. These alterations were associated with increase in tubulointerstitial lesions and macrophage number, reduction of endothelial cells, and increased VEGF, vimentin, and alpha-smooth muscle actin expression in the outer renal medulla in the SED+CP group. We also found increased levels of renal IL-1 beta and increased excretion of monocyte chemoattractant protein-1 and transforming growth factor-beta compared with controls. These changes were milder in trained rats, associated with increased levels of renal tissue NO, and increased expression of p-eNOS and stromal cell-derived factor-1 alpha (a chemokine involved in kidney repair) in the kidneys of CP-injected trained rats. Conclusions: The protective effect of previous training in CP-treated rats was associated with reduced endothelial cell lesions and increased renal production of NO in trained rats. (c) 2018 The Author(s) Published by S. Karger AG, Basel. (AU)

• Processo FAPESP: 13/23113-5 - Influência do condicionamento físico prévio na transdiferenciação de células dos túbulos renais de ratos com lesão renal provocada por cisplatina
• Beneficiário: Lucas Chaves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/50180-2 - Evolução da lesão renal aguda e crônica em ratos submetidos ao pré-condicionamento físico: participação do endotélio
• Beneficiário: Heloísa Della Coletta Francescato
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores