Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes

Autor(es)

Frade, Maria Lucia ^[1] ; de Annunzio, Sarah Raquel ^[1] ; Fioramonti Calixto, Giovana Maria ^[2] ; Victorelli, Francesca Damiani ^[2] ; Chorilli, Marlus ^[2] ; Fontana, Carla Raquel ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 23, n. 2 FEB 2018.
• Citações Web of Science: 6

Resumo

Chitosan (CH) is a biopolymer that exhibits a number of interesting properties such as anti-inflammatory and antibacterial activity and is also a promising platform for the incorporation of photosensitizing agents. This study aimed to evaluate the efficacy of antimicrobial activity of chitosan hydrogel formulation alone and in combination with the methylene blue (MB) associated with antimicrobial photodynamic therapy (aPDT) against planktonic and biofilm phase of Propionibacterium acnes. Suspensions were sensitized with 12.5, 25.0, 37.5, 50.0 mu g/mL of MB for 10 min and biofilms to 75, 100 and 150 mu g/mL for 30 min then exposed to red light (660 nm) at 90 J/cm(2) and 150 J/cm(2) respectively. After treatments, survival fractions were calculated by counting the number of colony-forming units. The lethal effect of aPDT associated with CH hydrogel in planktonic phase was achieved with 12.5 mu g/mL MB and 1.9 log10 biofilm reduction using 75 mu g/mL MB. Rheological studies showed that formulations exhibited pseudoplastic non-Newtonian behavior without thixotropy. Bioadhesion test evidenced that the formulations are highly adhesive to skin and the incorporation of MB did not influence the bioadhesive force of the formulations. (AU)

• Processo FAPESP: 16/05345-4 - Otimização da terapia fotodinâmica para doenças infecciosas utilizando o peptídeo aureína 1.2
• Beneficiário: Carla Raquel Fontana
• Modalidade de apoio: Auxílio à Pesquisa - Regular