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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enhancement of colon carcinogenesis by the combination of indole-3 carbinol and synbiotics in hemin-fed rats

Texto completo
Autor(es):
de Moura, Nelci A. [1] ; Caetano, Brunno F. R. [1] ; de Moraes, Leonardo N. [1] ; Carvalho, Robson F. [1] ; Rodrigues, Maria A. M. [2] ; Barbisan, Luis F. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Morphol, BR-18618689 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Med, Dept Pathol, BR-18610307 Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Food and Chemical Toxicology; v. 112, p. 11-18, FEB 2018.
Citações Web of Science: 1
Resumo

The risk of developing colorectal cancer (CRC) could be associated with red and processed meat intake. Experimental data supports that hemin iron, found abundantly in red meat, promotes CRC in mice and rats, while indole-3 carbinol (I3C) and synbiotics (syn) exert anti-carcinogenic activities in most studies of colon carcinogenesis. This study aimed to investigate the modifying effects of I3C and syn (inulin + Bifidobacterium lactis), given separately or together, on dimethylhidrazine (DMH)-induced colon carcinogenesis in hemin-fed rats. All animals were given four subcutaneous DMH injections and then, two weeks after carcinogen exposure, they began a basal diet containing hemin, hemin + I3C, hemin + syn, or hemin + I3C + syn for 23 weeks. The combination of I3C + syn significantly increased fecal water genotoxicity, tumor volume and invasiveness when compared to the hemin-fed control group. The groups fed I3C or syn alone had a significant reduction in the number of preneoplastic aberrant crypt foci (ACF) lesions compared to the hemin-fed group. Dietary I3C also reduced fecal water genotoxicity. Gene expression analysis of colorectal tumors demonstrated that the combination of dietary I3C + syn increased transcript levels for Raf1 and decreased tumor progression and invasiveness related to the genes Cdh1 and Appl1. This analysis also revealed that the Tnf and Cdh1 genes were significantly up- and down-regulated, respectively, in tumors of rats that received I3C, in comparison with the hemin-fed group. These findings reveal that the joint administration of I3C and syn enhanced the development of colon tumors induced by DMH in hemin-fed rats, while they potentially reduced ACF development when given alone. (AU)

Processo FAPESP: 11/23699-4 - Efeitos da ingestão de simbiótico e indol 3- carbinol sobre o processo de carcinogênese química de cólon em Ratos Wistar alimentados com dieta contendo heme
Beneficiário:Nelci Antunes de Moura
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/12631-2 - Efeitos de simbióticos e indol 3-carbinol na expressão de genes relacionados à carcinogênese de cólon em ratos alimentados com dieta contendo heme
Beneficiário:Luís Fernando Barbisan
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/08033-5 - Genotoxicidade da água fecal e desenvolvimento de focos de criptas aberrantes em Ratos Wistar alimentados com heme, Indol-3-Carbinol e/ou simbiótico
Beneficiário:Brunno Felipe Ramos Caetano
Linha de fomento: Bolsas no Brasil - Iniciação Científica