| Texto completo | |
| Autor(es): |
Mantovani, Cinthia de Carvalho
[1]
;
Pereira e Silva, Jefferson
[1]
;
Forster, Guilherme
[1]
;
de Almeida, Rafael Menck
[1]
;
de Albuquerque Diniz, Edna Maria
[2]
;
Yonamine, Mauricio
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, 13B, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Hosp Sao Paulo, Pediat Dept, Av Prof Lineu Prestes 2565, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1074, p. 1-7, FEB 1 2018. |
| Citações Web of Science: | 4 |
| Resumo | |
Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Delta 9-tetrahydrocannabinol-9carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography-mass spectrometry (GC MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were < 8.4% for all concentration levels. Accuracy was in 101.7-108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure. (AU) | |
| Processo FAPESP: | 11/14108-2 - Uso da extração acelerada por solvente (ASE) para determinação cromatográfica de analitos de cocaína e tetraidrocanabinol em amostras de mecônio |
| Beneficiário: | Cinthia de Carvalho Mantovani |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/15700-3 - Avaliação da exposição fetal e infantil à cocaína e canabinoides por meio de análise toxicológica em leite materno, mecônio e tecido do cordão umbilical |
| Beneficiário: | Mauricio Yonamine |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/13079-4 - Determinação cromatográfica de analitos de tetraidrocanabinol em amostras de mecônio |
| Beneficiário: | Guilherme Forster |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |