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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Simultaneous accelerated solvent extraction and hydrolysis of 11-nor-Delta(9)-tetrahydrocannabino1-9-carboxylic acid glucuronide in meconium samples for gas. chromatography-mass spectrometry analysis

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Autor(es):
Mantovani, Cinthia de Carvalho [1] ; Pereira e Silva, Jefferson [1] ; Forster, Guilherme [1] ; de Almeida, Rafael Menck [1] ; de Albuquerque Diniz, Edna Maria [2] ; Yonamine, Mauricio [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, 13B, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Hosp Sao Paulo, Pediat Dept, Av Prof Lineu Prestes 2565, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1074, p. 1-7, FEB 1 2018.
Citações Web of Science: 4
Resumo

Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Delta 9-tetrahydrocannabinol-9carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography-mass spectrometry (GC MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were < 8.4% for all concentration levels. Accuracy was in 101.7-108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure. (AU)

Processo FAPESP: 11/14108-2 - Uso da extração acelerada por solvente (ASE) para determinação cromatográfica de analitos de cocaína e tetraidrocanabinol em amostras de mecônio
Beneficiário:Cinthia de Carvalho Mantovani
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 15/15700-3 - Avaliação da exposição fetal e infantil à cocaína e canabinoides por meio de análise toxicológica em leite materno, mecônio e tecido do cordão umbilical
Beneficiário:Mauricio Yonamine
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/13079-4 - Determinação cromatográfica de analitos de tetraidrocanabinol em amostras de mecônio
Beneficiário:Guilherme Forster
Linha de fomento: Bolsas no Brasil - Iniciação Científica