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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simultaneous accelerated solvent extraction and hydrolysis of 11-nor-Delta(9)-tetrahydrocannabino1-9-carboxylic acid glucuronide in meconium samples for gas. chromatography-mass spectrometry analysis

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Author(s):
Mantovani, Cinthia de Carvalho [1] ; Pereira e Silva, Jefferson [1] ; Forster, Guilherme [1] ; de Almeida, Rafael Menck [1] ; de Albuquerque Diniz, Edna Maria [2] ; Yonamine, Mauricio [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, 13B, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Hosp Sao Paulo, Pediat Dept, Av Prof Lineu Prestes 2565, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1074, p. 1-7, FEB 1 2018.
Web of Science Citations: 4
Abstract

Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Delta 9-tetrahydrocannabinol-9carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography-mass spectrometry (GC MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were < 8.4% for all concentration levels. Accuracy was in 101.7-108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure. (AU)

FAPESP's process: 15/15700-3 - Evaluation of fetal and infant exposure to cocaine and cannabinoids by toxicological analysis in breast milk, meconium and umbilical cord tissue
Grantee:Mauricio Yonamine
Support type: Regular Research Grants
FAPESP's process: 11/14108-2 - Accelerated solvent extraction (ASE) for chromatographic analysis of cocaine and tetrahydrocannabinol analytes in meconium samples
Grantee:Cinthia de Carvalho Mantovani
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/13079-4 - Chromatographic analysis of cocaine and tetrahydrocannabinol analytes in meconium samples
Grantee:Guilherme Forster
Support type: Scholarships in Brazil - Scientific Initiation