Simultaneous accelerated solvent extraction and hydrolysis of 11-nor-Delta(9)-tetrahydrocannabino1-9-carboxylic acid glucuronide in meconium samples for gas. chromatography-mass spectrometry analysis

Author(s)

Mantovani, Cinthia de Carvalho ^[1] ; Pereira e Silva, Jefferson ^[1] ; Forster, Guilherme ^[1] ; de Almeida, Rafael Menck ^[1] ; de Albuquerque Diniz, Edna Maria ^[2] ; Yonamine, Mauricio ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes 580, 13B, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Hosp Sao Paulo, Pediat Dept, Av Prof Lineu Prestes 2565, BR-05508900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1074, p. 1-7, FEB 1 2018.
• Web of Science Citations: 4

Abstract

Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Delta 9-tetrahydrocannabinol-9carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography-mass spectrometry (GC MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were < 8.4% for all concentration levels. Accuracy was in 101.7-108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure. (AU)

• FAPESP's process: 11/14108-2 - Accelerated solvent extraction (ASE) for chromatographic analysis of cocaine and tetrahydrocannabinol analytes in meconium samples
• Grantee: Cinthia de Carvalho Mantovani
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/15700-3 - Evaluation of fetal and infant exposure to cocaine and cannabinoids by toxicological analysis in breast milk, meconium and umbilical cord tissue
• Grantee: Mauricio Yonamine
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/13079-4 - Chromatographic analysis of cocaine and tetrahydrocannabinol analyses in meconium samples
• Grantee: Guilherme Forster
• Support Opportunities: Scholarships in Brazil - Scientific Initiation