Effects of telmisartan and losartan on irradiated testes

Autor(es)

Mansano, Naira da Silva ^[1] ; Jorge, Isabela Fernandes ^[2] ; Chies, Agnaldo Bruno ^[3] ; Viani, Gustavo Arruda ^[4] ; Spadella, Maria Angelica ^[5]

Afiliação do(s) autor(es)

^[1] Marilia Med Sch, Marilia, SP - Brazil

^[2] Inst Med Assistance State Level Govt Employees IA, Sao Paulo, SP - Brazil

^[3] Marilia Med Sch, Lab Pharmacol, Marilia, SP - Brazil

^[4] Univ Sao Paulo, Oncol & Radiotherapy, Ribeirao Preto, SP - Brazil

^[5] Marilia Med Sch, Human Embryol Lab, Marilia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 194, p. 157-167, FEB 1 2018.
• Citações Web of Science: 4

Resumo

Aims: To analyze the effects of radiation on the reproductive tissue of male Wistar rats and to evaluate whether treatment with the Ang II AT1 receptor antagonists telmisartan and losartan mitigate the dysfunctions resulting from this exposure. Main methods: Rats were randomly divided into groups: Control, Irradiated, Telmisartan, Losartan, Irradiated + Telmisartan, and Irradiated + Losartan. Single dose of 5Gy was administered directly into the scrotum, followed by treatment with telmisartan (12 mg/kg/day) or losartan (34 mg/kg/two times/day) for 60 days. Testicular function parameters were evaluated from spermatozoa of the vas deferens. Testes were processed for histopathological and morphometric-stereological analysis. Proliferating cell nuclear antigen (PCNA) immunohistochemistry was evaluated. Key findings: Radiation significantly reduced sperm motility, concentration, vitality, and increased the number of abnormal spermatozoa. Telmisartan and losartan did not significantly prevent these radiation-induced disorders. Seminiferous tubules were atrophied in both untreated and treated irradiated testes, and exhibited vacuoles, increased interstitial tissue and high number of blood vessels. However, several seminiferous tubules in recuperation were founded among damaged tubules in the testes of treated animals. The PCNA immunohistochemistry confirmed these outcomes. PCNA-positive cells were detected in dividing spermatogonia and spermatocytes from irradiated telmisartan and losartan treated rats whereas in the only-irradiated group, PCNA staining was observed in the nuclei of only the surviving spermatogonia. (AU)

• Processo FAPESP: 15/20740-4 - Ação dos antagonistas do receptor AT1 na mitigação de danos estruturais e funcionais em tecido reprodutivo de ratos expostos à radiação ionizante
• Beneficiário: Maria Angélica Spadella Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11843-4 - Análise morfológica e morfométrica-estereológica de testículos irradiados versus o potencial efeito protetor de inibidores do sistema renina-angiotensina
• Beneficiário: Isabela Fernandes Jorge
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica