Simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography-tandem mass spectrometry system

Autor(es)

Lopes, Bianca Rebelo ^[1] ; Cassiano, Neila Maria ^[1] ; Carvalho, Daniela Miarelli ^[2] ; Dantas Moises, Elaine Christine ^[2] ; Cass, Quezia Bezerra ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Quim, Nucleo Pesquisa Cromatog, SEPARARE, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Dept Ginecol & Obstet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmaceutical and Biomedical Analysis; v. 150, p. 362-367, FEB 20 2018.
• Citações Web of Science: 5

Resumo

A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC-MS/MS) was employed for the determination of fluoxetine (FLU) and norfluoxetine (N-FLU) in colostrum and mature milk by direct sample injection. With a run time of 12 min representing a gain in throughput analysis, the validated methods furnished selectivity, extraction efficiency, accuracy, and precision in accordance with the criteria preconized by the European Medicines Agency guidelines. With a linear range of 3.00-150 ng/mL for FLU and 4.00-200 ng/mL for N-FLU they were applied to the analysis of colostrum and mature milk samples from nursing mothers. The paper discusses the differences and similarity of sample preparation for this two sample matrices. The herein reported methods are an advance in sample preparation procedures providing waste reduction and a sustainable approach. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/17138-0 - Métodos para análises de pequenas moléculas em fluidos biológicos: o uso de colunas de meios de acesso restrito para injeção direta de amostras
• Beneficiário: Quezia Bezerra Cass
• Modalidade de apoio: Auxílio à Pesquisa - Regular