| Full text | |
| Author(s): |
Lopes, Bianca Rebelo
[1]
;
Cassiano, Neila Maria
[1]
;
Carvalho, Daniela Miarelli
[2]
;
Dantas Moises, Elaine Christine
[2]
;
Cass, Quezia Bezerra
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, Nucleo Pesquisa Cromatog, SEPARARE, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Dept Ginecol & Obstet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Pharmaceutical and Biomedical Analysis; v. 150, p. 362-367, FEB 20 2018. |
| Web of Science Citations: | 5 |
| Abstract | |
A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC-MS/MS) was employed for the determination of fluoxetine (FLU) and norfluoxetine (N-FLU) in colostrum and mature milk by direct sample injection. With a run time of 12 min representing a gain in throughput analysis, the validated methods furnished selectivity, extraction efficiency, accuracy, and precision in accordance with the criteria preconized by the European Medicines Agency guidelines. With a linear range of 3.00-150 ng/mL for FLU and 4.00-200 ng/mL for N-FLU they were applied to the analysis of colostrum and mature milk samples from nursing mothers. The paper discusses the differences and similarity of sample preparation for this two sample matrices. The herein reported methods are an advance in sample preparation procedures providing waste reduction and a sustainable approach. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 09/17138-0 - Methods for quantification of small molecules in biological fluids: the use of restricted access media columns for direct injection of samples |
| Grantee: | Quezia Bezerra Cass |
| Support Opportunities: | Regular Research Grants |