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Methods for quantification of small molecules in biological fluids: the use of restricted access media columns for direct injection of samples


The solution to the paradigm of getting efficiency in less time of analysis, in the context of the High Performance Liquid Chromatography, separations have been explored with success in 2D. The multidimensional analyses are used for a wide range of complex matrices and/or samples. In the preparation of samples, the separations in two dimensions are carried out by coupling restricted access media columns to achirals and/or chiral columns, for a variety of classes of compounds in different matrices. The use of taden mass spectrometry hyphenated to liquid chromatography has expanded the realms of the use of liquid chromatography for quantification of small molecules in complex samples matrices. In this project, the multidimensional chromatography will be employed for quantification of small molecules in biological fluids by direct injection of samples. The project is part of an ongoing project in the group and expands the use of restricted access media bovine serum albumin (RAM-BSA) columns for the two complexes matrices: human urine and milk and also on the use of RAM-BSA ionic columns for quantification of amphoteric compounds in human urine and bovine milk. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
WACHTER, NAIHARA; AQUINO, JOSE MARIO; DENADAI, MARINA; BARREIRO, JULIANA C.; SILVA, ADILSON JOSE; CASS, QUEZIA B.; BOCCHI, NERILSO; ROCHA-FILHO, ROMEU C. Electrochemical degradation of the antibiotic ciprofloxacin in a flow reactor using distinct BDD anodes: Reaction kinetics, identification and toxicity of the degradation products. Chemosphere, v. 234, p. 461-470, NOV 2019. Web of Science Citations: 0.
WACHTER, NAIHARA; AQUINO, JOSE M.; DENADAI, MARINA; BARREIRO, JULIANA C.; SILVA, ADILSON J.; CASS, QUEZIA B.; ROCHA-FILHO, ROMEU C.; BOCCHI, NERILSO. Optimization of the electrochemical degradation process of the antibiotic ciprofloxacin using a double-sided beta-PbO2 anode in a flow reactor: kinetics, identification of oxidation intermediates and toxicity evaluation. Environmental Science and Pollution Research, v. 26, n. 5, p. 4438-4449, FEB 2019. Web of Science Citations: 6.
LOPES, BIANCA REBELO; CASSIANO, NEILA MARIA; CARVALHO, DANIELA MIARELLI; DANTAS MOISES, ELAINE CHRISTINE; CASS, QUEZIA BEZERRA. Simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography-tandem mass spectrometry system. Journal of Pharmaceutical and Biomedical Analysis, v. 150, p. 362-367, FEB 20 2018. Web of Science Citations: 5.
CASSIANO, NEILA M.; BARREIRO, JULIANA C.; CASS, QUEZIA B. New Trends in Sample Preparation in Brazil: an Overview of Bioanalytical Applications by Liquid Chromatography. Journal of the Brazilian Chemical Society, v. 25, n. 1, p. 9-19, JAN 2014. Web of Science Citations: 9.
MOURA, FRANCIANE; DE ALMEIDA, FERNANDO G.; LOPES, BIANCA REBELO; CASS, QUEZIA BEZERRA. Quantification of ampicillin in bovine milk by coupled-column ultrahigh-performance liquid chromatography-tandem mass spectrometry. JOURNAL OF SEPARATION SCIENCE, v. 35, n. 19, p. 2615-2620, OCT 2012. Web of Science Citations: 12.
LOURENCO, TIAGO C.; BATISTA, JR., JOAO M.; FURLAN, MAYSA; HE, YANAN; NAFIE, LAURENCE A.; SANTANA, CESAR C.; CASS, QUEZIA B. Albendazole sulfoxide enantiomers: Preparative chiral separation and absolute stereochemistry. Journal of Chromatography A, v. 1230, p. 61-65, MAR 23 2012. Web of Science Citations: 19.

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