Advanced search
Start date
Betweenand

Development of selective phases for in-tube SPME/LC-MS/MS analysis of parabens and drugs in biological samples

Abstract

In recent years, modern analytical chemistry has seeked simplification by hyphenation of the techniques, automation (high-throughput performance), and miniaturization of the analytical systems. In this context, the in-tube solid phase microextration (in-tube SPME) hyphenated to liquid chromatography coupled to mass spectrometry, tandem system (LC-MS/MS, reference analytical technique for bioanalysis) can be highlighted. The development of stationary phases for in-tube SPME, such as the restricted access materials (RAM), the molecularly imprinted polymers (MIP), and hybrid silica monolith have enabled direct biological fluid introduction into chromatographic systems and selective pre-concentration of the analytes, respectively. In this project, the phases MIP-RAM and monolithic hybrid silica containing cyano and phenyl functional groups will be synthesized for in-tube SPME/LC-MS/MS analyses of parabens in breast milk samples and of drugs in plasma samples from schizophrenic patients, respectively. The proposed objectives emerged from two distinct public health problems. The first one concerns to research carried out in vitro and in vivo, which have demonstrated that parabens, a chemical preservative present in cosmetic products, can mimic the action of estrogen and induce the growth of MCF7 cells, which are related to the development of breast cancer. Further than this fact, in lactating women, breast milk may be the major route for the excretion of bioaculative lipophilic substances (such as parabens). The second problem concerns to schizophrenia, considered as the most serious of the neuropsychiatric upheavals. Apart from antipsychotics, great part of the schizophrenic patients also makes concomitant use of other classes of drugs, such as antidepressants, anticonvulsants, and anxiolytics, in order to decrease the symptoms associated with this condition. Consequently, the analysis of these drugs in plasma samples from schizophrenic patients is important to adjust the doses, minimize adverse effects and check the patient compliance with treatment. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, ISRAEL D.; MELO, LIDERVAN P.; JARDIM, ISABEL C. S. F.; MONTEIRO, JULIANA C. S.; NAKANO, ANA MARCIA S.; QUEIROZ, MARIA EUGENIA C. Selective molecularly imprinted polymer combined with restricted access material for in-tube SPME/UHPLC-MS/MS of parabens in breast milk samples. Analytica Chimica Acta, v. 932, p. 49-59, AUG 17 2016. Web of Science Citations: 31.
MIRANDA, LUIS FELIPPE C.; DOMINGUES, DIEGO S.; QUEIROZ, MARIA EUGENIA C. Selective solid-phase extraction using molecularly imprinted polymers for analysis of venlafaxine, O-desmethylvenlafaxine, and N-desmethylvenlafaxine in plasma samples by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1458, p. 46-53, AUG 5 2016. Web of Science Citations: 12.
DOMINGUES, DIEGO SOARES; LEMOS PINTO, MONIA APARECIDA; DE SOUZA, ISRAEL DONIZETI; CECILIO HALLAK, JAIME EDUARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; COSTA QUEIROZ, MARIA EUGENIA. Determination of Drugs in Plasma Samples by High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Therapeutic Drug Monitoring of Schizophrenic Patients. JOURNAL OF ANALYTICAL TOXICOLOGY, v. 40, n. 1, p. 28-36, JAN-FEB 2016. Web of Science Citations: 11.
DE SOUZA, ISRAEL D.; DOMINGUES, DIEGO S.; QUEIROZ, MARIA E. C. Hybrid silica monolith for microextraction by packed sorbent to determine drugs from plasma samples by liquid chromatography-tandem mass spectrometry. Talanta, v. 140, p. 166-175, AUG 1 2015. Web of Science Citations: 26.
DOMINGUES, DIEGO SOARES; DE SOUZA, ISRAEL DONIZETI; COSTA QUEIROZ, MARIA EUGENIA. Analysis of drugs in plasma samples from schizophrenic patients by column-switching liquid chromatography-tandem mass spectrometry with organic-inorganic hybrid cyanopropyl monolithic column. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 993, p. 26-35, JUL 1 2015. Web of Science Citations: 18.
DOMINGUES, DIEGO SOARES; CREVELIN, EDUARDO JOSE; BERALDO DE MORAES, LUIZ ALBERTO; CECILIO HALLAK, JAIME EDUARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; COSTA QUEIROZ, MARIA EUGENIA. Simultaneous determination of amino acids and neurotransmitters in plasma samples from schizophrenic patients by hydrophilic interaction liquid chromatography with tandem mass spectrometry. JOURNAL OF SEPARATION SCIENCE, v. 38, n. 5, p. 780-787, MAR 2015. Web of Science Citations: 16.
JARDIM, VALERIA CRISTINA; MELO, LIDERVAN DE PAULA; DOMINGUES, DIEGO SOARES; COSTA QUEIROZ, MARIA EUGENIA. Determination of parabens in urine samples by microextraction using packed sorbent and ultra-performance liquid chromatography coupled to tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 974, p. 35-41, JAN 1 2015. Web of Science Citations: 17.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.