New functional aspects of eicosanoids

• Grant number: 15/00658-1
• Support Opportunities: Multi-user Equipment Program
• Start date: March 01, 2016
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Lúcia Helena Faccioli
• Grantee: Lúcia Helena Faccioli
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/07125-6 - New functional aspects of eicosanoids, AP.TEM
• EMU web page: http://fcfrp.usp.br/centro-de-excelencia-de-quantificacao-e-identifica/#.XOgWnWxYZPY
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatografia líquida acoplada a espectrômetro de massa
• Manufacturer: AB Sciex
• Model: Nexera-TripleTOF 5600+ (LC-MS/MS)

Abstract

Mass spectrometry is a highly sensitive, selective and rapid technique for characterizing multiple species of lipids, simultaneously, without the need for prior isolation and derivatization of compounds of interest. Coupled to ultra-pressure liquid chromatography (UHPLC-MS) is a powerful tool for identification, characterization and quantification of lipid mediators in complex matrices, allowing identification of lipid regiosomers. Lipidomics is the identification and quantification of lipids in biological systems, being the same, as a tool to evaluate the profile of certain lipid classes in immune and inflammatory responses. In our projects, we will focus our analysis to the class of eicosanoids, membrane phospholipids and neutral lipids. The formation of the Centre of Excellence for Quantification and Identification of Lipids (CEQIL), justifies the request of UHPLC-MS/MS system for this purpose. So, by being the first Lipid Analysis Center, using this technology in Brazil, this equipment (mass spectrometer) will have multiuser character, allowing the insertion of the quantification and identification of lipids in various interest projects such as biotechnology, clinical and basic research. So even being targeted primarily to analysis of a class of biomolecules (lipids), the use of the equipment will be constant and will have high demand, allowing the generation of new knowledge, scientific articles of impact and technological advances. (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SORGI, CARLOS ARTERIO; SOARES, ELYARA MARIA; ROSADA, ROGERIO SILVA; BITENCOURT, CLAUDIA SILVA; ZOCCAL, KARINA FURLANI; TARTARI PEREIRA, PRISCILLA APARECIDA; FONTANARI, CAROLINE; BRANDAO, IZAIRA; MASSON, ANA PAULA; RAMOS, SIMONE GUSMAO; et al. Eicosanoid pathway on host resistance and inflammation during Mycobacterium tuberculosis infection is comprised by LTB4 reduction but not PGE(2) increment. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1866, n. 3, MAR 1 2020. (10/11239-6, 15/00658-1, 09/07169-5)

ACUNHA, TANIZE; NARDINI, VIVIANI; FERRANTI PETI, ANA PAULA; BORGES PRADO, MORGANA KELLY; BERALDO MORAES, LUIZ ALBERTO; FACCIOLI, LUCIA HELENA. Targeted analysis of eicosanoids derived from cytochrome P450 pathway by high-resolution multiple-reaction monitoring mass spectrometry. Journal of Mass Spectrometry, v. 56, n. 7, JUL 2021. (16/16788-4, 15/00658-1, 14/07125-6, 18/25704-4)

ZOCCAL, KARINA F.; GARDINASSI, LUIZ G.; BORDON, KARLA C. F.; ARANTES, ELIANE C.; MARLEAU, SYLVIE; ONG, HUY; FACCIOLI, LUCIA H.. EP80317 Restrains Inflammation and Mortality Caused by Scorpion Envenomation in Mice. FRONTIERS IN PHARMACOLOGY, v. 10, MAR 1 2019. (14/07125-6, 14/03332-7, 15/00658-1)

SOUZA, CAMILA O. S.; ESPINDOLA, MILENA S.; FONTANARI, CAROLINE; PRADO, MORGANA K. B.; FRANTZ, FABIANI G.; RODRIGUES, VANDERLEI; GARDINASSI, LUIZ G.; FACCIOLI, LUCIA H.. CD18 Regulates Monocyte Hematopoiesis and Promotes Resistance to Experimental Schistosomiasis. FRONTIERS IN IMMUNOLOGY, v. 9, AUG 31 2018. (14/07125-6, 15/00658-1)

DOS SANTOS, RAFAELA SILVA; SORGI, CARLOS ARTERIO; FERRANTI PETI, ANA PAULA; VERAS, FLAVIO PROTASIO; FACCIOLI, LUCIA HELENA; GALDINO, GIOVANE. Involvement of Spinal Cannabinoid CB2 Receptors in Exercise-Induced Antinociception. Neuroscience, v. 418, p. 177-188, OCT 15 2019. (14/07125-6, 15/00658-1)

CHAVES, JAISA O.; SANCHES, VITOR L.; VIGANO, JULIANE; MESQUITA, LEONARDO M. DE SOUZA; DE SOUZA, MARIANA C.; DA SILVA, LAISE C.; ACUNHA, TANIZE; FACCIOLI, LUCIA H.; ROSTAGNO, MAURICIO A.. Integration of pressurized liquid extraction and in-line solid-phase extraction to simultaneously extract and concentrate phenolic compounds from lemon peel (Citrus limon L.). Food Research International, v. 157, p. 12-pg., 2022-07-01. (13/04304-4, 20/08421-9, 15/00658-1, 19/13496-0, 18/14582-5, 20/15774-5)

ESTEVES, ELIANE; BIZZARRO, BRUNA; COSTA, FRANCISCO BORGES; RAMIREZ-HERNANDEZ, ALEJANDRO; FERRANTI PETI, ANA PAULA; DEPIERI CATANEO, ALLAN HENRIQUE; WOWK, PRYSCILLA FANINI; TIMOTEO, RODOLFO PESSATO; LABRUNA, MARCELO BAHIA; SILVA JUNIOR, PEDRO ISMAEL; et al. Amblyomma sculptum Salivary PGE(2) Modulates the Dendritic Cell-Rickettsia rickettsii Interactions in vitro and in vivo. FRONTIERS IN IMMUNOLOGY, v. 10, FEB 4 2019. (15/00658-1, 13/26450-2)

FERRANTI PETI, ANA PAULA; LOCACHEVIC, GISELE APARECIDA; BORGES PRADO, MORGANA KELLY; BERALDO DE MORAES, LUIZ ALBERTO; FACCIOLI, LUCIA HELENA. High-resolution multiple reaction monitoring method for quantification of steroidal hormones in plasma. Journal of Mass Spectrometry, v. 53, n. 5, p. 423-431, MAY 2018. (14/07125-6, 16/16788-4, 15/00658-1)

ACUNHA, TANIZE; NARDINI, VIVIANI; FACCIOLI, LUCIA HELENA. A lipidomics approach reveals new insights intoCrotalus durissus terrificusandBothrops moojenisnake venoms. ARCHIVES OF TOXICOLOGY, v. 95, n. 1, SEP 2020. (15/00658-1, 14/07125-6, 18/25704-4)

LOUREIRO, LUMA DA COSTA; LOUREIRO, LUANA DA COSTA; GABRIEL-JUNIOR, EDSON ALVES; ZAMBUZI, FABIANA ALBANI; FONTANARI, CAROLINE; SALES-CAMPOS, HELIOSWILTON; FRANTZ, FABIANI GAI; FACCIOLI, LUCIA HELENA; SORGI, CARLOS ARTERIO. Pulmonary surfactant phosphatidylcholines induce immunological adaptation of alveolar macrophages. Molecular Immunology, v. 122, p. 163-172, JUN 2020. (18/15066-0, 14/07125-6, 15/00658-1)

FUZO, CARLOS A.; FRAGA-SILVA, THAIS F. C.; MARUYAMA, SANDRA R.; BASTOS, VICTOR A. F.; ROGERIO, LUANA A.; TAKAMIYA, NAYORE T.; DA SILVA-NETO, PEDRO V.; PIMENTEL, VINICIUS E.; TORO, DIANA M.; PEREZ, MALENA M.; et al. The turning point of COVID-19 severity is associated with a unique circulating neutrophil gene signature. IMMUNOLOGY, v. 169, n. 3, p. 21-pg., 2023-03-08. (15/00658-1, 21/04590-3, 20/08534-8, 20/05207-6, 19/09881-6, 14/07125-6, 20/05270-0, 17/16328-6)

PRADO, MORGANA K. B.; FONTANARI, CAROLINE; SOUZA, CAMILA O. S.; GARDINASSI, LUIZ G.; ZOCCAL, KARINA F.; DE PAULA-SILVA, FRANCISCO W. G.; PETI, ANA P. F.; SORGI, CARLOS A.; MEIRELLES, ALYNE F. G.; RAMOS, SIMONE G.; et al. IL-22 Promotes IFN-gamma-Mediated Immunity against Histoplasma capsulatum Infection. BIOMOLECULES, v. 10, n. 6, JUN 2020. (13/08216-2, 14/07125-6, 15/00658-1)

DOS REIS, MOUZARLLEM BARROS; FACCIOLI, LUCIA HELENA. CARDIAC DYSFUNCTION INDUCED BY SCORPION ENVENOMATION IS MEDIATED BY NLRP3-INDUCED ACUTE MYOCARDITIS. Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/02314-3, 15/00658-1, 14/07125-6)

ACUNHA, TANIZE; ROCHA, BRUNO ALVES; NARDINI, VIVIANI; BARBOSA JR, FERNANDO; FACCIOLI, LUCIA HELENA. Lipidomic profiling of the Brazilian yellow scorpion venom: new insights into inflammatory responses following Tityus serrulatus envenomation. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 86, n. 9, p. 13-pg., 2023-03-11. (15/00658-1, 21/03633-0, 18/24069-3, 14/07125-6, 22/07979-1, 18/25704-4)

DE OLIVEIRA, HERICK ULISSES; DOS SANTOS, RAFAELA SILVA; SILVA MALTA, IAGO HENRIQUE; PINHO, JOSE PHELLIPE; SANTOS ALMEIDA, ANA FLAVIA; SORGI, CARLOS ARTERIO; FERRANTI PETI, ANA PAULA; XAVIER, GABRIELA SANTOS; DOS REIS, LUCIANA MARIA; FACCIOLI, LUCIA HELENA; et al. Investigation of the Involvement of the Endocannabinoid System in TENS-Induced Antinociception. JOURNAL OF PAIN, v. 21, n. 7-8, p. 820-835, JUL-AUG 2020. (14/07125-6, 15/00658-1)

PEREIRA, PRISCILLA A. T.; BITENCOURT, CLAUDIA S.; REIS, MOUZARLLEM B.; FRANTZ, FABIANI G.; SORGI, CARLOS A.; SOUZA, CAMILA O. S.; SILVA, CELIO L.; GARDINASSI, LUIZ G.; FACCIOLI, LUCIA H.. Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation. Inflammation Research, v. 69, n. 1, NOV 2019. (07/04741-4, 14/07125-6, 09/07169-5, 15/00658-1)

REIS, MOUZARLLEM B.; RODRIGUES, FERNANDA L.; LAUTHERBACH, NATALIA; KANASHIRO, ALEXANDRE; SORGI, CARLOS A.; MEIRELLES, ALYNE F. G.; SILVA, CARLOS A. A.; ZOCCAL, KARINA F.; SOUZA, CAMILA O. S.; RAMOS, SIMONE G.; et al. Interleukin-1 receptor-induced PGE(2) production controls acetylcholine-mediated cardiac dysfunction and mortality during scorpion envenomation. NATURE COMMUNICATIONS, v. 11, n. 1, OCT 28 2020. (15/21976-1, 14/07125-6, 17/02314-3, 15/00658-1)

SORGI, CARLOS ARTERIO; FERRANTI PETI, ANA PAULA; PETTA, TANIA; GALVAO MEIRELLES, ALYNE FAVERO; FONTANARI, CAROLINE; BERALDO DE MORAES, LUIZ ALBERTO; FACCIOLI, LUCIA HELENA. Comprehensive high-resolution multiple-reaction monitoring mass spectrometry for targeted eicosanoid assays. SCIENTIFIC DATA, v. 5, AUG 21 2018. (14/07125-6, 16/16788-4, 15/00658-1)

LOCACHEVIC, GISELE A.; PRADO, MORGANA K. B.; ZOCCAL, KARINA F.; PEREIRA, PRISCILLA A. T.; SORGI, CARLOS A.; BORTOLANZA, MARIZA; PETI, ANA PAULA F.; FOGACA, MANOELA V.; GUIMARAES, FRANCISCO S.; DEL BEL, ELAINE; et al. Paradoxical Effect of LTB4 on the Regulation of Stress-Induced Corticosterone Production. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, v. 13, APR 16 2019. (14/07125-6, 15/00658-1)

PAULA, T. D.; SILVA, B. R.; GRANDO, M. D.; SOUZA, H. C. D.; BENDHACK, L. M.. Activation of TP receptors induces high release of PGI(2) in coronary arteries of renal hypertensive rats. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, v. 122, p. 125-133, SEP 2018. (15/00658-1, 15/17080-2)

ZOCCAL, KARINA F.; GARDINASSI, LUIZ G.; SORGI, CARLOS A.; MEIRELLES, ALYNE F. G.; BORDON, KARLA C. F.; GLEZER, ISAIAS; CUPO, PALMIRA; MATSUNO, ALESSANDRA K.; BOLLELA, VALDES R.; ARANTES, ELIANE C.; et al. CD36 Shunts Eicosanoid Metabolism to Repress CD14 Licensed Interleukin-1 beta Release and Inflammation. FRONTIERS IN IMMUNOLOGY, v. 9, APR 27 2018. (13/07937-8, 14/07125-6, 15/00658-1, 14/03332-7)

SORGI, CARLOS A.; ZARINI, SIMONA; MARTIN, SARAH A.; SANCHEZ, RAPHAEL L.; SCANDIUZZI, RODRIGO F.; GIJON, MIGUEL A.; GUIJAS, CARLOS; FLAMAND, NICOLAS; MURPHY, ROBERT C.; FACCIOLI, LUCIA H.. Dormant 5-lipoxygenase in inflammatory macrophages is triggered by exogenous arachidonic acid. SCIENTIFIC REPORTS, v. 7, SEP 8 2017. (14/07125-6, 14/13127-1, 15/00658-1)

DE CARVALHO, JONATAN C. S.; DA SILVA-NETO, PEDRO V.; TORO, DIANA M.; FUZO, CARLOS A.; NARDINI, VIVIANI; PIMENTEL, VINICIUS E.; PEREZ, MALENA M.; FRAGA-SILVA, THAIS F. C.; OLIVEIRA, CAMILLA N. S.; DEGIOVANI, AUGUSTO M.; et al. The Interplay among Glucocorticoid Therapy, Platelet-Activating Factor and Endocannabinoid Release Influences the Inflammatory Response to COVID-19. Viruses-Basel, v. 15, n. 2, p. 22-pg., 2023-02-01. (15/00658-1, 21/04590-3, 20/08534-8, 20/05207-6, 14/07125-6, 22/07287-2, 20/05270-0)

MONARA FERREIRA DE SOUSA, ANDRADE YASMIN; DE CASTRO, MONARA VIERA; TAVARES, VICTOR DE SOUZA; SOUZA, RAYANE DA SILVA OLIVEIRA; FACCIOLI, LUCIA HELENA; LIMA, JONILSON BERLINK; SORGI, CARLOS ARTERIO; BORGES, VALERIA M.; ARAUJO-SANTOS, THEO. Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes. Memórias do Instituto Oswaldo Cruz, v. 118, p. 7-pg., 2023-01-01. (15/00658-1)

DE ALMEIDA, FELIPE CAMPOS; BERZOTI-COELHO, MARIA G.; TORO, DIANA MOTA; CACEMIRO, MAIRA DA COSTA; BASSAN, VITOR LEONARDO; BARRETTO, GABRIEL DESSOTTI; MARQUES GARIBALDI, PEDRO MANOEL; PALMA, LEONARDO CARVALHO; DE FIGUEIREDO-PONTES, LORENA LOBO; SORGI, CARLOS ARTERIO; et al. Bioactive Lipids as Chronic Myeloid Leukemia's Potential Biomarkers for Disease Progression and Response to Tyrosine Kinase Inhibitors. FRONTIERS IN IMMUNOLOGY, v. 13, p. 8-pg., 2022-04-13. (14/50947-7, 15/21866-1, 18/19714-7, 15/23555-3, 15/00658-1, 13/08135-2, 18/01756-5)

ALBORNOZ, SANDRA P. PALMA; FRAGA-SILVA, THAIS F. C.; DE CARVALHO, RENAN V. H.; RODRIGUES, TAMARA S.; GEMBRE, ANA FLAVIA; DE OLIVEIRA, ROMULO SILVA; DE SOUZA, FERNANDA MESQUITA; CORREA, GISELI FURLAN; RAMALHO, LEANDRA N. Z.; CARLOS, DANIELA; et al. Cell death induced by NLRP3-palmitate axis impairs pulmonary damage tolerance and aggravates immunopathology during obesity-tuberculosis comorbidity. JOURNAL OF PATHOLOGY, v. 259, n. 3, p. 13-pg., 2023-01-03. (17/21629-5, 15/00658-1, 15/00774-1)

DE MACEDO, LUANA HENRIQUE; SOUZA, CAMILA OLIVEIRA SILVA; GARDINASSI, LUIZ GUSTAVO; FACCIOLI, LUCIA HELENA. CD14 regulates the metabolomic profiles of distinct macrophage subsets under steady and activated states. Immunobiology, v. 227, n. 2, p. 10-pg., 2022-02-19. (14/07125-6, 18/10929-0, 15/00658-1, 18/22667-0)