Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans

Texto completo
Autor(es):
Rocha, Guilherme Roncari [1] ; Florez Salamanca, Elkin Jahir [1] ; de Barros, Ana Leticia [1] ; Vitorino Lobo, Carmelia Isabel [1] ; Klein, Marlise Inez [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 18, FEB 14 2018.
Citações Web of Science: 9
Resumo

Background: Dental caries is considered a multifactorial disease, in which microorganisms play an important role. The diet is decisive in the biofilm formation because it provides the necessary resources for cellular growth and exopolysaccharides synthesis. Exopolysaccharides are the main components of the extracellular matrix (ECM). The ECM provides a 3D structure, support for the microorganisms and form diffusion-limited environments (acidic niches) that cause demineralization of the dental enamel. Streptococcus mutans is the main producer of exopolysaccharides. Candida albicans is detected together with S. mutans in biofilms associated with severe caries lesions. Thus, this study aimed to determine the effect of tt-farnesol and myricetin topical treatments on cariogenic biofilms formed by Streptococcus mutans and Candida albicans. Methods: In vitro dual-species biofilms were grown on saliva-coated hydroxyapatite discs, using tryptone-yeast extract broth with 1% sucrose (37 degrees C, 5% CO2). Twice-daily topical treatments were performed with: vehicle (ethanol 15%, negative control), 2 mM myricetin, 4 mM tt-farnesol, myricetin + tt-farnesol, myricetin + tt-farnesol + fluoride (250 ppm), fluoride, and chlorhexidine digluconate (0.12%; positive control). After 67 h, biofilms were evaluated to determine biofilm biomass, microbial population, and water-soluble and -insoluble exopolysaccharides in the ECM. Results: Only the positive control yielded a reduced quantity of biomass and microbial population, while tt-farnesol treatment was the least efficient in reducing C. albicans population. The combination therapy myricetin + farnesol + fluoride significantly reduced water-soluble exopolysaccharides in the ECM (vs. negative control; p < 0.05; ANOVA one-way, followed by Tukey's test), similarly to the positive control. Conclusions: Therefore, the combination therapy negatively influenced an important virulence trait of cariogenic biofilms. However, the concentrations of both myricetin and tt-farnesol should be increased to produce a more pronounced effect to control these biofilms. (AU)

Processo FAPESP: 15/03424-1 - Avaliação da eficácia de agentes antimicrobianos liberados por novo sistema de liberação controlada de fármacos contra biofilmes orais patogênicos
Beneficiário:Guilherme Roncari Rocha
Modalidade de apoio: Bolsas no Brasil - Mestrado