Propionibacterium acnes Enhances the Immunogenicity of HIVBr18 Human Immunodeficiency Virus-1 Vaccine

Autor(es)

Teixeira, Daniela ^[1] ; Ishimura, Mayari Eika ^[1] ; Apostolico, Juliana de Souza ^[1] ; Viel, Jacqueline Miyuki ^[1] ; Passarelli, Victor Cabelho ^[1] ; Cunha-Neto, Edecio ^[2] ; Rosa, Daniela Santoro ^[1] ; Longo-Maugeri, Ieda Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Immunol, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Clin Immunol & Allergy LIM60, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 9, FEB 7 2018.
• Citações Web of Science: 2

Resumo

Immunization of BALB/c mice with HIVBr18, a DNA vaccine containing 18 CD4(+) T cell epitopes from human immunodeficiency virus (HIV), induced specific CD4(+) and CD8(+) T cell responses in a broad, polyfunctional and persistent manner. With the aim of increasing the immunogenicity of this vaccine, the effect of Propionibacterium acnes as an adjuvant was evaluated. The adjuvant effects of this bacterium have been extensively demonstrated in both experimental and clinical settings. Herein, administration of two doses of HIVBr18, in the presence of P. acnes, increased the proliferation of HIV-1-specific CD4(+) and CD8(+) T lymphocytes, the polyfunctional profile of CD4(+) T cells, the production of IFN-gamma, and the number of recognized vaccine-encoded peptides. One of the bacterial components responsible for most of the adjuvant effects observed was a soluble polysaccharide extracted from the P. acnes cell wall. Furthermore, within 10 weeks after immunization, the proliferation of specific T cells and production of IFN-gamma were maintained when the whole bacterium was administered, demonstrating a greater effect on the longevity of the immune response by P. acnes. Even with fewer immunization doses, P. acnes was found to be a potent adjuvant capable of potentiating the effects of the HIVBr18 vaccine. Therefore, P. acnes may be a potential adjuvant to aid this vaccine in inducing immunity or for therapeutic use. (AU)

• Processo FAPESP: 14/25307-4 - Efeito adjuvante da Propionibacterium acnes e de sua fração polissacarídica purificada sobre a imunogenicidade de uma vacina para o vírus da imunodeficiência humana tipo 1 (HIV-1)
• Beneficiário: Ieda Maria Longo Maugéri
• Modalidade de apoio: Auxílio à Pesquisa - Regular