pH changes of mixed biofilms of Streptococcus mutans and Candida albicans after exposure to sucrose solutions in vitro

Autor(es)

Cavazana, Thamires Priscila ^[1] ; Pessan, Juliano Pelim ^[1] ; Hosida, Thayse Yumi ^[1] ; Monteiro, Douglas Roberto ^[2] ; Botazzo Delbem, Alberto Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Sao Paulo - Brazil

^[2] Univ Western Sao Paulo UNOESTE, GPD, BR-19050920 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 90, p. 9-12, JUN 2018.
• Citações Web of Science: 1

Resumo

Objective: This study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans. Design: Biofilms were developed for 96 h, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 min. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 min after removal. Results: Sucrose solutions at 10 and 20% required 1 min to significantly reduce the biofilm pH, while for 30% sucrose a significant reduction was already seen immediately after its removal, even for the shortest exposure time. For an exposure of 3 min to 20% sucrose, the biofilm pH attained the critical value for hydroxyapatite dissolution when measured 1 min after sucrose removal, followed by a recovery phase. Conclusions: A mixed biofilm of S. mutans and C. albicans exposed to a 20% sucrose solution for 3 min exhibited a pattern of pH change similar to that observed in vivo, despite at a higher speed when compared to in vivo conditions. (AU)

• Processo FAPESP: 16/01752-4 - Efeito do trimetafosfato de sódio, associado ou não ao fluoreto, no biofilme total e fluido do biofilme formado in vitro
• Beneficiário: Thamires Priscila Cavazana
• Modalidade de apoio: Bolsas no Brasil - Mestrado