Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

pH changes of mixed biofilms of Streptococcus mutans and Candida albicans after exposure to sucrose solutions in vitro

Full text
Author(s):
Cavazana, Thamires Priscila [1] ; Pessan, Juliano Pelim [1] ; Hosida, Thayse Yumi [1] ; Monteiro, Douglas Roberto [2] ; Botazzo Delbem, Alberto Carlos [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Sao Paulo - Brazil
[2] Univ Western Sao Paulo UNOESTE, GPD, BR-19050920 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 90, p. 9-12, JUN 2018.
Web of Science Citations: 1
Abstract

Objective: This study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans. Design: Biofilms were developed for 96 h, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 min. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 min after removal. Results: Sucrose solutions at 10 and 20% required 1 min to significantly reduce the biofilm pH, while for 30% sucrose a significant reduction was already seen immediately after its removal, even for the shortest exposure time. For an exposure of 3 min to 20% sucrose, the biofilm pH attained the critical value for hydroxyapatite dissolution when measured 1 min after sucrose removal, followed by a recovery phase. Conclusions: A mixed biofilm of S. mutans and C. albicans exposed to a 20% sucrose solution for 3 min exhibited a pattern of pH change similar to that observed in vivo, despite at a higher speed when compared to in vivo conditions. (AU)

FAPESP's process: 16/01752-4 - Effect of sodium trimetaphosphate, associated or not with fluoride, in the total biofilm and biofilm fluid formed in vitro
Grantee:Thamires Priscila Cavazana
Support Opportunities: Scholarships in Brazil - Master