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Effect of dentifrices containing sodium trimetaphasphate, xylitol and erythritol, associated or not to fluoride, on mixed biofilms of S. mutans and C. albicans formed in vitro

Grant number: 18/26204-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2019
Effective date (End): December 31, 2019
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Juliano Pelim Pessan
Grantee:Tamires Passadori Martins
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Xylitol and Erythritol are compounds of natural origin, regarded as potential sources of therapeutic agents, and which can be used in the control of dental caries. However, the mechanism by which these compounds act in biofilm and in the fluid biofilm is still uncertain. Thus, the present study aims to assess the the effect of dentifrices containing sodium trimetaphosphate (TMP), xylitol, erythritol, and fluoride (F), alone or in different associations, on planktonic cells and on dual-species biofilms of S. mutans and C. albicans formed in vitro. Dentifrices containing TMP (0.2%), F (200 ppm), xylitol (16%) and erythritol (4%), alone or in combination, will be prepared, in addition to dentifrices containing 1100 ppm F (positive control) or placebo (negative control), totaling 11 study groups. The effect of the treatments on planktonic cells will be assessed by the determination of the minimum inhibitory concentration, using the broth microdilution method. The biofilms will be formed into wells of microtiter plates, containing 200 µL of the inoculum of the microorganisms in mixed culture of a suspension containing 108 e 107 cells/mL, respectively for S. mutans and C. albicans. Biofilms will be treated twice daily for 1 minute and once again the next day, with slurries of each dentifrice. After this period, the anti-biofilm effect of the treatments will be quantified by counting the colony forming units (CFU), total biofilm biomass (CV staining method), metabolic activity of the cells (XTT reduction method and Resazurin). All assays will be performed in triplicate, on three different occasions. Data will be analyzed regarding their distribution (normality and homogeneity) before the determination of appropriate statistical test. If the use of parametric tests is possible, the data will be analyzed by ANOVA and Tukey's test. Otherwise, Kruskal-Wallis' and Dunn's tests will be used. The level of significance adopted will be 5%.