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Evaluation of the effect of solutions containing Malva sylvestris extract on microcosm biofilm and on enamel demineralization prevention

Grant number: 21/03053-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2021
Effective date (End): May 31, 2022
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Ana Carolina Magalhães
Grantee:Larissa Ribeiro Alves da Silva
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


The aim of this study will be to evaluate the effect of solutions containing different concentrations of Malva sylvestris on the CFU counts for cariogenic microorganisms present in microcosm biofilm as well as on the prevention of enamel demineralization. Firstly, we will determine MIC and MBC, testing different concentrations of Malva sylvestris extract on Streptococcus mutans. The MIC and MBC values will be tested on microcosm biofilm model. For this, 99 bovine enamel samples will be prepared and distributed into the following experimental groups 1) Malva sylvestris (MIC); 2) Malva sylvestris (MBC); 3) Malva sylvestris (MIC) + 5% xylitol; 4) Malva sylvestris (MBC) + 5% xylitol; 5) Malva sylvestris (MIC) + 5% xylitol + Fluoride (250 ppm); 6) Malva sylvestris (MBC) + 5% xylitol + Fluoride (250 ppm); 7) Xylitol 5%; 8) Fluoride (250 ppm); 9) Malva sylvestris (Malvatricin Plus® - Positive control 2); 10) Chlorhexidine (Positive control 1) and 11) PBS (Negative control) (n = 9). For the formation of the microcosm biofilm, a pool of saliva collected from 10 healthy individuals will be used. In 48-well plates, each enamel sample will be exposed to 750 µL of inoculum (human saliva-glycerol + McBain saliva, 1:50), for 8 h. After 8 h, the inoculum will be removed, the samples will be washed with PBS (5 s), and then they will receive 750 µL of fresh medium (McBain artificial saliva) with 0.2% sucrose for 16 h, completing 24 h. From the 2nd to the 5th day, the samples will be treated with the experimental solutions for 1 minute and the medium containing 0.2% sucrose will be changed daily. Three samples per group will be tested in each of the three biological replicates (final n = 9). The response variables will be: UFC count for Streptococcus mutans/S. sobrinus and Lactobacillus sp.; and quantification of enamel demineralization by transverse microradiography (TMR). The data will be submitted to the appropriate statistical analysis (parametric or non-parametric test), considering p<0.05.

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