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Evaluation of the effect of different experimental and commercial natural extracts in the form of toothpastes and oral mouthrinses on the viability and activity of microcosm biofilm and on enamel demineralization prevention in vitro

Grant number: 17/17249-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2018
Status:Discontinued
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Ana Carolina Magalhães
Grantee:Aline Silva Braga
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):18/26506-1 - Effect of different experimental toothpastes and oral mouthrinses containing natural extracts on the relative metabolic activity of microcosm biofilm and on enamel demineralization prevention in vitro, BE.EP.DR

Abstract

In the recent years, the demand for natural extracts that can be added to dental products has been increased for the purpose of controlling the most frequent oral problems. Accordingly, this work aims to evaluate the effect of experimental and commercial dentifrices and mouthrinses containing different natural extracts on the viability and activity of microcosm biofilm as well as in the prevention of enamel demineralization in vitro. Initially, the values of MIC and MBC will be determined for three extract from Vochysia tucanorum Mart. and Myrcia bella Cambess. leaves and Matricaria chamomilla L. flower in aqueous and hydroalcoholic solution (5% ethanol) on S. mutans (ATCC 25175) and total human salivary microorganisms. In the second step, these extracts will be tested in the forms of solution and dentifrice and compared with commercial products containing natural agents with respect to the antimicrobial and anticaries effect using a microcosm biofilm model. For this purpose, saliva pool from 10 health individuals (70% saliva and 30% glycerol) will be mixed with McBain artificial saliva (with 0.2% sucrose) for the biofilm formation. Bovine enamel samples (4 mm x 4 mm) will be submitted to microcosm biofilm formation for 5 days, which will be treated daily (1x60s / day) with solution or dentifrice. With respect to solutions, we will test three experimental and three commercial ones: 1. Vochysia tucanorum Mart., 2. Myrcia bella Cambess., 3. Matricaria chamomilla L, 4. Orgânico natural®-Contente/Uberlândia-Brazil, 5. Boni Natural Menta & Malaleuca®-Boni/São Bernardo do Campo-Brazil, 6. Malvatricin Plus®-Daudt/Rio de Janeiro-Brazil, 7. PerioGard®-Colgate-Palmolive/São Paulo-Brazil (positive control) and 8. PBS (negative control). With respect to dentifrices, we will test three experimental and three commercial ones: 1. Vochysia tucanorum Mart., 2. Myrcia bella Cambess., 3. Matricaria chamomilla L, 4. Orgânico natural®-Contente/Uberlândia-Brazil, 5. Boni Natural Menta & Malaleuca®-Boni/São Bernardo do Campo-Brazil, 6. Propolis & Myrrh®-Tom's Maine-Kennebunk-USA, 7. Colgate Total 12 Clean Mint®-Colgate-Palmolive/São Paulo-Brazil (positive control) and 8. placebo dentifrice (negative control). The response variables will be: 1) percentages of live and dead bacteria and the presence of extracellular polysaccharides by fluorescence using confocal microscopy; 2) acidogenicity of the biofilm by measuring the presence of lactic acid using enzymatic method; 3) colony forming units (CFUs) counting for total microorganisms, total streptococci, total lactobacilli, Streptococcus mutans and Streptococcus sobrinus or 4) detection and analysis of relative metabolic activity (%) of total bacteria, S. mutans, S. sobrinus, Lactobacillus (genus), Bifidobacterium dentium, Scardovia wiggsiae and Fusobacteria (genus) by qPCR; 5) quantification of enamel demineralization using transverse microradiography. The data will be submitted to the appropriate statistical analysis (parametric or non-parametric test), considering p <0.05.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRAGA, ALINE SILVA; GIROTTI, LETICIA DRAGONETTI; DE MELO SIMAS, LETICIA LOBO; PIRES, JULIANA GONCALVES; PELA, VINICIUS TAIOQUI; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Effect of commercial herbal toothpastes and mouth rinses on the prevention of enamel demineralization using a microcosm biofilm model. BIOFOULING, v. 35, n. 7, p. 796-804, AUG 9 2019. Web of Science Citations: 1.

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