Evaluation of the antimicrobial effect of alternative and commercial agents on a microcosm biofilm and on prevention of dental demineralization

• Grant number: 17/00556-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: April 30, 2020
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Ana Carolina Magalhães
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The present study aims to evaluate the antimicrobial and anticaries effect of different commercial agents (natural toothpastes and natural mouthrinses) and alternative (natural extracts and experimental fluoride varnish) in respect to the following parameters: minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradicating concentration (MBEC), microbiological viability (MTT test and/or fluorescence assay using confocal microscopy), production of lactic acid and extracellular and intracellular polysaccharides (EPS/IPS) and/or CFUs counting using a monospecie biofilm (S. mutans) or cariogenic microcosm biofilm formed on enamel and/or dentin, which will be analyzed in terms of mineral loss using transverse microradiography (TMR). All experiments involve individual students' projects, which will take part of this regular grant proposal. In assay 1, commercial toothpastes containing natural agents will be evaluated using microcosm biofilm on enamel in respect microbial viability by fluorescence, production of EPS/IPS and lactate and counting of CFU for cariogenic microorganisms (A.S.B.). In assay 2, commercial mouthrinses containing natural agents will be evaluated using microcosm biofilm on enamel in respect microbial viability by fluorescence, production of EPS/IPS and lactate and counting of CFU for cariogenic microorganisms (A.S.B.). In assay 3, we will evaluate the effect of natural extracts (Myracrodruon urundeuva All and Qualea grandiflora Mart) in respect to MIC, MBC, MBIC and MBEC for S. mutans as well as the viability of S. mutans biofilm formed on enamel by fluorescence (S.S.Z). In assay 4, the same natural extracts will be evaluated using a microcosm biofilm model on enamel, with the analysis of viability using MTT and fluorescence assays, production of EPS/IPS and lactate and counting of CFU for cariogenic microorganisms (J.G.P.). In assay 5, fluoride varnishes (TiF4 and NaF) will be evaluated using microcosm biofilm on enamel and dentin in respect to fluorescence viability, production of EPS/IPS and lactate and counting of CFU for cariogenic microorganisms (D.M.S.S). For all assays, the prevention of demineralization will be quantified using TMR. The data will be submitted to the statistical analysis (p<0.05). (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES DOS SANTOS, DAIANA M.; BRAGA, ALINE S.; RIZK, MARTA; WIEGAND, ANNETTE; MAGALHAES, ANA C.. Comparison between micro-computed tomography and transverse microradiography of sound dentine treated with fluorides and demineralized by microcosm biofilm. European Journal of Oral Sciences, OCT 2019. (17/13386-5, 17/00556-0)

PIRES, JULIANA GONCALVES; ZABINI, SARA SALUSTIANO; BRAGA, ALINE SILVA; FABRIS, RITA DE CASSIA; DE ANDRADE, FLAVIANA BOMBARDA; DE OLIVEIRA, RODRIGO CARDOSO; MAGALHAES, ANA CAROLINA. Hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves on Streptococcus mutans biofilm and tooth demineralization. ARCHIVES OF ORAL BIOLOGY, v. 91, p. 17-22, JUL 2018. (17/00556-0, 16/20212-0)

BRAGA, ALINE SILVA; GIROTTI, LETICIA DRAGONETTI; DE MELO SIMAS, LETICIA LOBO; PIRES, JULIANA GONCALVES; PELA, VINICIUS TAIOQUI; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Effect of commercial herbal toothpastes and mouth rinses on the prevention of enamel demineralization using a microcosm biofilm model. BIOFOULING, v. 35, n. 7, p. 796-804, AUG 9 2019. (17/00556-0, 17/23165-6, 17/23013-1, 17/17249-2)

BRAGA, ALINE SILVA; ABDELBARY, MOHAMED MOSTAFA HEFNY; KIM, RAFAELA RICCI; DE SOUZA ROSA DE MELO, FERNANDA PEREIRA; SALDANHA, LUIZ LEONARDO; DOKKEDAL, ANNE LIGIA; CONRADS, GEORG; ESTEVES-OLIVEIRA, MARCELLA; MAGALHAES, ANA CAROLINA. The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development. ANTIBIOTICS-BASEL, v. 11, n. 3, p. 14-pg., 2022-03-01. (17/00556-0, 17/17249-2, 19/21797-0, 18/26506-1)

SOARES DOS SANTOS, DAIANA M.; BRAGA, ALINE S.; RIZK, MARTA; WIEGAND, ANNETTE; MAGALHAES, ANA C.. Comparison between micro-computed tomography and transverse microradiography of sound dentine treated with fluorides and demineralized by microcosm biofilm. European Journal of Oral Sciences, v. 127, n. 6, p. 7-pg., 2019-10-04. (17/13386-5, 17/00556-0)

SOARES DOS SANTOS, DAIANA MORELI; PIRES, JULIANA GONCALVES; SILVA, ALINE BRAGA; ARANDA SALOMAO, PRISCILA MARIA; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Protective Effect of 4% Titanium Tetrafluoride Varnish on Dentin Demineralization Using a Microcosm Biofilm Model. Caries Research, v. 53, n. 5, p. 576-583, 2019. (17/00556-0, 16/11152-4)

PIRES, JULIANA GONCALVES; BRAGA, ALINE SILVA; DE ANDRADE, FLAVIANA BOMBARDA; SALDANHA, LUIZ LEONARDO; DOKKEDAL, ANNE LIGIA; DE OLIVEIRA, RODRIGO CARDOSO; MAGALHAES, ANA CAROLINA. Effect of hydroalcoholic extract of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves on the viability and activity of microcosm biofilm and on enamel demineralization. Journal of Applied Oral Science, v. 27, 2019. (17/00556-0)

BRAGA, ALINE SILVA; SIMAS, LETICIA LOBO DE MELO; PIRES, JULIANA GONSALVES; SOUZA, BEATRIZ MARTINES; MELO, FERNANDA PEREIRA DE SOUZA ROSA DE; SALDANHA, LUIZ LEONARDO; DOKKEDAL, ANNE LIGIA; MAGALHAES, ANA CAROLINA. Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel. Journal of Dentistry, v. 99, AUG 2020. (17/00556-0, 17/17249-2, 17/23165-6)

BRAGA, ALINE SILVA; DEGAND, GIOVANA BISSOLI; PIRES, JULIANA GONCALVES; SOARES DOS SANTOS, DAIANA MOREL; MAGALHAES, ANA CAROLINA. Effect of oral antimicrobial mouthrinses containing alcohol on viability of Streptococcus mutans and microcosm biofilm and on the prevention of enamel caries lesions. AMERICAN JOURNAL OF DENTISTRY, v. 31, n. 3, p. 121-125, JUN 2018. (17/00556-0, 16/20915-1)