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Evaluation of the antimicrobial effect of 4% titanium tetrafluoride varnish in a cariogenic microcosm biofilm model on dentine

Grant number: 16/11152-4
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2016
Effective date (End): August 17, 2018
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Ana Carolina Magalhães
Grantee:Daiana Moreli Soares dos Santos
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):17/13386-5 - Effect of 4% titanium tetrafluoride varnish on the prevention of dentin demineralization under a cariogenic microcosm biofilm model - micro-CT and TMR analysis, BE.EP.MS


In a previous study, our research group demonstrated that 4% TiF4 varnish did not show antimicrobial effect in a microcosm biofilm on enamel; however, it was able to decrease enamel demineralization. Therefore, the present study aims to evaluate the effect of this agent on the microbial viability, acid and extracellular polysaccharide (PEC) production as well as on the microorganism counting of a cariogenic microcosm biofilm produced on root dentine. For the microcosm biofilm formation, saliva will be collected from 10 healthy subjects, who must have not brush their teeth for the last 24h and not drink or eat for the last 2h. The saliva will be diluted (70% saliva and 30% glycerol), and aliquots of 1ml will be stored at -80ºC. Sample of bovine dentine (4mm x 4mm) will be prepared and treated for 6h with the varnishes containing: A) 4% TiF4 (pH 1.0, 2.45% F); B) 5.42% NaF (pH 5.0, 2.45% F); C) placebo D) 2% Chlorexidine gel - positive control or E) untreated - negative control (n=4 x biological triplicate/assay). These samples will be plated in 24-wells plates and exposed to the human saliva mixed with McBain saliva (1:50) with 0.2% sucrose for the microcosm biofilm formation. In the Assay 1, the live and dead bacteria will be evidenced by fluorescence using a confocal microscope. In the Assay 2, acidogenicity will be evaluated, by measuring the lactic acid using enzymatic methods and microplate reader. In the Assay 3, the quantity of PEC production will be evaluated by colorimetric method using microplate reader. In the Assay 4, the microbial suspension removed from the biofilm will be diluted and spread in Agar plates for colony forming unit (CFU) counting for total microorganism, total streptococcus, mutans streptococcus and total lactobacillus and in the Assay 5, the dentine demineralization will be measured using transverse microradiography. The data will be plotted and submitted to the statistical analyses (p<0.05). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES DOS SANTOS, DAIANA MORELI; PIRES, JULIANA GONCALVES; SILVA, ALINE BRAGA; ARANDA SALOMAO, PRISCILA MARIA; RABELO BUZALAF, MARILIA AFONSO; MAGALHAES, ANA CAROLINA. Protective Effect of 4% Titanium Tetrafluoride Varnish on Dentin Demineralization Using a Microcosm Biofilm Model. Caries Research, v. 53, n. 5, p. 576-583, 2019. Web of Science Citations: 1.
SOARES DOS SANTOS, DAIANA MORELI; PIPES, JULIANA GONCALVES; BRAGA, ALINE SILVA; ARANDA SALOMAO, PRISCILA MARIA; MAGALHAES, ANA CAROLINA. Comparison between static and semi-dynamic models for microcosm biofilm formation on dentin. Journal of Applied Oral Science, v. 27, 2019. Web of Science Citations: 0.

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