ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments

Autor(es):Mostrar menos -

Barbosa, Natalia S. ^[1] ; Mendonca, Leila R. ^{[1, 2]} ; Dias, Marcos V. S. ^{[1, 3]} ; Pontelli, Marjorie C. ^[1] ; da Silva, Elaine Z. M. ^[3] ; Criado, Miria F. ^[1] ; da Silva-Januario, Mara E. ^[1] ; Schindler, Michael ^[4] ; Jamur, Maria C. ^[3] ; Oliver, Constance ^[3] ; Arruda, Eurico ^{[1, 3]} ; daSilva, Luis L. P. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Ctr Virus Res, Ribeirao Preto, SP - Brazil

^[2] Oswaldo Cruz Fdn FIOCRUZ, Dept Virol & Expt Therapy LAVITE, IAM, Recife, PE - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol, Ribeirao Preto, SP - Brazil

^[4] Univ Hosp Tubingen, Mol Virol Human Infect Dis, Inst Med Virol & Epidemiol, Tubingen - Germany

• Tipo de documento: Artigo Científico
• Fonte: PLOS PATHOGENS; v. 14, n. 5 MAY 2018.
• Citações Web of Science: 3

Resumo

Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance in public heath very little is known about the host cell factors hijacked by these viruses to support assembly and cell egress. Here we show that assembly of Oropouche virus, a member of the genus Orthobunyavirus that causes a frequent arboviral infection in South America countries, involves budding of virus particles toward the lumen of Golgi cisternae. As viral replication progresses, these Golgi subcompartments become enlarged and physically separated from Golgi stacks, forming Oropouche viral factory (Vfs) units. At the ultrastructural level, these virally modified Golgi cisternae acquire an MVB appearance, and while they lack typical early and late endosome markers, they become enriched in endosomal complex required for transport (ESCRT) proteins that are involved in MVB biogenesis. Further microscopy and viral replication analysis showed that functional ESCRT machinery is required for efficient Vf morphogenesis and production of infectious OROV particles. Taken together, our results indicate that OROV attracts ESCRT machinery components to Golgi cisternae to mediate membrane remodeling events required for viral assembly and budding at these compartments. This represents an unprecedented mechanism of how viruses hijack host cell components for coordinated morphogenesis. (AU)

• Processo FAPESP: 09/54014-7 - EMU: aquisição dos equipamentos IVIS Spectrum e microscópio de excitação por dois fótons para imagem in vivo
• Beneficiário: Enilza Maria Espreafico
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 14/02438-6 - Estudos com Bunyaviridae causadores de doença
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/25812-0 - Estudo sobre as interações moleculares de HIV-1 com o sistema de endomembranas da célula hospedeira
• Beneficiário: Luis Lamberti Pinto da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular