Molecular phylogeny of Culex subgenus Melanoconion (Diptera: Culicidae) based on nuclear and mitochondrial protein-coding genes

Autor(es)

Torres-Gutierrez, Carolina ^{[1, 2]} ; de Oliveira, Tatiane M. P. ^[1] ; Emerson, Kevin J. ^[3] ; Bergo, Eduardo Sterlino ^[4] ; Mureb Sallum, Maria Anice ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Ave Doutor Arnaldo 715, BR-01246904 Sao Paulo - Brazil

^[2] Univ Antioquia, Fac Med, PECET, Calle 67 53-108, Medellin - Colombia

^[3] St Marys Coll Maryland, Biol Dept, St Marys City, MD 20686 - USA

^[4] Secretaria Estado Saude Sao Paulo, Superintendencia Controle Endemias, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ROYAL SOCIETY OPEN SCIENCE; v. 5, n. 5 MAY 2018.
• Citações Web of Science: 0

Resumo

The subgenus Melanoconion of the mosquito genus Culex is taxonomically diverse and is widely distributed in the Neotropical Region, with 10 species occurring in the Nearctic Region. Species of this subgenus pose a taxonomical challenge because morphological identification is based largely on anatomical characters of the male genitalia. We addressed the monophyly of the Spissipes and Melanoconion Sections of the subgenus Melanoconion and some of the informal groups in each section. Our sample taxa included 97 specimens representing 43 species, from which we analysed fragments of two single-copy nuclear genes (CAD, HB) and one mitochondrial gene (COI). Phylogenetic relationships within the subgenus are presented based on results of maximum-likelihood and Bayesian analyses using a multi-locus matrix of DNA sequences. We show a molecular phylogeny of Melanoconion in which both sections were recovered as monophyletic groups. The monophyly of the Atratus and Pilosus groups was confirmed. Within each section, other monophyletic groups were recovered highlighting the potential need for future nomenclature rearrangement. The phylogenetic signal contained in nuclear genes, when analysed together, was more informative than each gene analysed separately, corroborating monophyly of Melanoconion relative to Culex (Culex) species included in the analyses, the Melanoconion and Spissipes Sections and some species groups. Our results provide new information for the classification of the subgenus and additional data that can be used to improve species identification when a more representative taxon sampling is available. (AU)

• Processo FAPESP: 14/26229-7 - Genômica de paisagens em gradientes latitudinais e ecologia de Anopheles darlingi
• Beneficiário: Maria Anice Mureb Sallum
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/18089-5 - Filogenia molecular do subgênero Melanoconion do gênero Culex (Diptera: Culicidae)
• Beneficiário: Carolina Torres Gutierrez
• Modalidade de apoio: Bolsas no Brasil - Doutorado