Metabolic responses of Eucalyptus species to different temperature regimes

Autor(es)

Mokochinski, Joao Benhur ^{[1, 2]} ; Mazzafera, Paulo ^[1] ; Helena Frankland Sawaya, Alexandra Christine ^{[1, 3]} ; Mumm, Roland ^[2] ; Hendricus de Vos, Ric Cornelis ^[2] ; Hall, Robert David ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, BR-13083862 Campinas, SP - Brazil

^[2] Wageningen Univ & Res, Wageningen Plant Res, Biosci, Wageningen - Netherlands

^[3] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, BR-13083862 Campinas, SP - Brazil

^[4] Wageningen Univ & Res, Lab Plant Physiol, Wageningen - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF INTEGRATIVE PLANT BIOLOGY; v. 60, n. 5, p. 397-411, MAY 2018.
• Citações Web of Science: 9

Resumo

Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may affect the metabolism of three species of Eucalyptus, E. dunnii, E. grandis and E. pellita. Young plants were grown for 53 d in the greenhouse and then transferred to growth chambers at 10 degrees C, 20 degrees C or 30 degrees C for another 7 d. In all three species the leaf chlorophyll content was positively correlated to temperature, and in E. pellita the highest temperature also resulted in a significant increase in stem biomass. Comprehensive metabolomics was performed using untargeted gas chromatography mass spectrometry (GC-MS) and liquid chromatography (LC)-MS. This approach enabled the comparison of the relative abundance of 88 polar primary metabolites from GC-MS and 625 semi-polar secondary metabolites from LC-MS. Using principal components analysis, a major effect of temperature was observed in each species which was larger than that resulting from the genetic background. Compounds mostly affected by temperature treatment were subsequently selected using partial least squares discriminant analysis and were further identified. These putative annotations indicated that soluble sugars and several polyphenols, including tannins, triterpenes and alkaloids were mostly influenced. (AU)

• Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Beneficiário: Paulo Mazzafera
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
• Beneficiário: Paulo Mazzafera
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 13/21306-0 - Desenvolvimento de métodos analíticos por uplc-ms para o estudo do metaboloma de duas espécies de eucalipto expostas a variações de temperatura e concentração de co2
• Beneficiário: João Benhur Mokochinski
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06987-7 - Análise metabolômica untargeted do eucalipto: da aquisição dos dados à interpretação biológica
• Beneficiário: João Benhur Mokochinski
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado