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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Metabolic responses of Eucalyptus species to different temperature regimes

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Author(s):
Mokochinski, Joao Benhur [1, 2] ; Mazzafera, Paulo [1] ; Helena Frankland Sawaya, Alexandra Christine [1, 3] ; Mumm, Roland [2] ; Hendricus de Vos, Ric Cornelis [2] ; Hall, Robert David [2, 4]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, BR-13083862 Campinas, SP - Brazil
[2] Wageningen Univ & Res, Wageningen Plant Res, Biosci, Wageningen - Netherlands
[3] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, BR-13083862 Campinas, SP - Brazil
[4] Wageningen Univ & Res, Lab Plant Physiol, Wageningen - Netherlands
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF INTEGRATIVE PLANT BIOLOGY; v. 60, n. 5, p. 397-411, MAY 2018.
Web of Science Citations: 9
Abstract

Species and hybrids of Eucalyptus are the world's most widely planted hardwood trees. They are cultivated across a wide range of latitudes and therefore environmental conditions. In this context, comprehensive metabolomics approaches have been used to assess how different temperature regimes may affect the metabolism of three species of Eucalyptus, E. dunnii, E. grandis and E. pellita. Young plants were grown for 53 d in the greenhouse and then transferred to growth chambers at 10 degrees C, 20 degrees C or 30 degrees C for another 7 d. In all three species the leaf chlorophyll content was positively correlated to temperature, and in E. pellita the highest temperature also resulted in a significant increase in stem biomass. Comprehensive metabolomics was performed using untargeted gas chromatography mass spectrometry (GC-MS) and liquid chromatography (LC)-MS. This approach enabled the comparison of the relative abundance of 88 polar primary metabolites from GC-MS and 625 semi-polar secondary metabolites from LC-MS. Using principal components analysis, a major effect of temperature was observed in each species which was larger than that resulting from the genetic background. Compounds mostly affected by temperature treatment were subsequently selected using partial least squares discriminant analysis and were further identified. These putative annotations indicated that soluble sugars and several polyphenols, including tannins, triterpenes and alkaloids were mostly influenced. (AU)

FAPESP's process: 15/06987-7 - Untargeted metabolomics of eucalyptus: from data acquisition to biological interpretation
Grantee:João Benhur Mokochinski
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
Grantee:Paulo Mazzafera
Support type: Research Grants - Research Partnership for Technological Innovation - PITE
FAPESP's process: 13/21306-0 - DEVELOPMENT OF ANALYTICAL METHODS BY UPLC-MS FOR THE METABOLOMIC STUDY OF TWO SPECIES OF EUCALYPTUS EXPOSED TO CHANGES IN TEMPERATURE AND CONCENTRATION OF CO2
Grantee:João Benhur Mokochinski
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Grantee:Paulo Mazzafera
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants