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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Field-deployable viral diagnostics using CRISPR-Cas13

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Autor(es):
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Myhrvold, Cameron [1, 2] ; Freije, Catherine A. [1, 2, 3] ; Gootenberg, Jonathan S. [4, 1, 5, 6, 7] ; Abudayyeh, Omar O. [4, 1, 6, 7, 8] ; Metsky, Hayden C. [9, 1] ; Durbin, Ann F. [10, 3] ; Kellner, Max J. [1] ; Tan, Amanda L. [11] ; Paul, Lauren M. [11] ; Parham, Leda A. [12] ; Garcia, Kimberly F. [12] ; Barnes, Kayla G. [1, 2, 13] ; Chak, Bridget [1, 2] ; Mondini, Adriano [14] ; Nogueira, Mauricio L. [15] ; Isern, Sharon [11] ; Michael, Scott F. [11] ; Lorenzana, Ivette [12] ; Yozwiak, Nathan L. [1, 2] ; MacInnis, Bronwyn L. [1, 13] ; Bosch, Irene [10, 16] ; Gehrke, Lee [10, 17, 3] ; Zhang, Feng [4, 1, 6, 7, 2] ; Sabeti, Pardis C. [1, 2, 3, 13, 18]
Número total de Autores: 24
Afiliação do(s) autor(es):
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[1] Broad Inst Massachusetts Inst Technol MIT & Harva, Cambridge, MA 02142 - USA
[2] Harvard Univ, Dept Organismal & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 - USA
[3] Harvard Med Sch, Div Med Sci, PhD Program Virol, Boston, MA 02115 - USA
[4] MIT, Dept Biol Engn, Cambridge, MA 02139 - USA
[5] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 - USA
[6] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 - USA
[7] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 - USA
[8] MIT, Dept Hlth Sci & Technol, Cambridge, MA 02139 - USA
[9] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 - USA
[10] MIT, Inst Med Engn & Sci, Cambridge, MA 02139 - USA
[11] Florida Gulf Coast Univ, Dept Biol Sci, Ft Myers, FL 33965 - USA
[12] Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Ctr Invest Genet, Tegucigalpa - Honduras
[13] Harvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 - USA
[14] Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara Lab Publ Hlth, Sao Paulo - Brazil
[15] Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Paulo - Brazil
[16] Mt Sinai Sch Med, Dept Med, New York, NY 10029 - USA
[17] Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 - USA
[18] HHMI, Chevy Chase, MD 20815 - USA
Número total de Afiliações: 18
Tipo de documento: Artigo Científico
Fonte: Science; v. 360, n. 6387, p. 444-448, APR 27 2018.
Citações Web of Science: 14
Resumo

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENVdetection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015-2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms. (AU)

Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Temático