| Full text | |
| Author(s): Show less - |
Myhrvold, Cameron
[1, 2]
;
Freije, Catherine A.
[1, 2, 3]
;
Gootenberg, Jonathan S.
[4, 1, 5, 6, 7]
;
Abudayyeh, Omar O.
[4, 1, 6, 7, 8]
;
Metsky, Hayden C.
[9, 1]
;
Durbin, Ann F.
[10, 3]
;
Kellner, Max J.
[1]
;
Tan, Amanda L.
[11]
;
Paul, Lauren M.
[11]
;
Parham, Leda A.
[12]
;
Garcia, Kimberly F.
[12]
;
Barnes, Kayla G.
[1, 2, 13]
;
Chak, Bridget
[1, 2]
;
Mondini, Adriano
[14]
;
Nogueira, Mauricio L.
[15]
;
Isern, Sharon
[11]
;
Michael, Scott F.
[11]
;
Lorenzana, Ivette
[12]
;
Yozwiak, Nathan L.
[1, 2]
;
MacInnis, Bronwyn L.
[1, 13]
;
Bosch, Irene
[10, 16]
;
Gehrke, Lee
[10, 17, 3]
;
Zhang, Feng
[4, 1, 6, 7, 2]
;
Sabeti, Pardis C.
[1, 2, 3, 13, 18]
Total Authors: 24
|
| Affiliation: Show less - | [1] Broad Inst Massachusetts Inst Technol MIT & Harva, Cambridge, MA 02142 - USA
[2] Harvard Univ, Dept Organismal & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 - USA
[3] Harvard Med Sch, Div Med Sci, PhD Program Virol, Boston, MA 02115 - USA
[4] MIT, Dept Biol Engn, Cambridge, MA 02139 - USA
[5] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 - USA
[6] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 - USA
[7] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 - USA
[8] MIT, Dept Hlth Sci & Technol, Cambridge, MA 02139 - USA
[9] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 - USA
[10] MIT, Inst Med Engn & Sci, Cambridge, MA 02139 - USA
[11] Florida Gulf Coast Univ, Dept Biol Sci, Ft Myers, FL 33965 - USA
[12] Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Ctr Invest Genet, Tegucigalpa - Honduras
[13] Harvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 - USA
[14] Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara Lab Publ Hlth, Sao Paulo - Brazil
[15] Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Paulo - Brazil
[16] Mt Sinai Sch Med, Dept Med, New York, NY 10029 - USA
[17] Harvard Med Sch, Dept Microbiol & Immunobiol, Boston, MA 02115 - USA
[18] HHMI, Chevy Chase, MD 20815 - USA
Total Affiliations: 18
|
| Document type: | Journal article |
| Source: | Science; v. 360, n. 6387, p. 444-448, APR 27 2018. |
| Web of Science Citations: | 82 |
| Abstract | |
Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENVdetection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015-2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms. (AU) | |
| FAPESP's process: | 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018 |
| Grantee: | Maurício Lacerda Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |