Insights about minority HIV-1 strains in transmitted drug resistance mutation dynamics and disease progression

Autor(es)

Leda, Ana Rachel ^[1] ; Hunter, James ^[1] ; Oliveira, Ursula Castro ^[2] ; Azevedo, Inacio Junqueira ^[2] ; Araripe Sucupira, Maria Cecilia ^[1] ; Diaz, Ricardo Sobhie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Infect Dis Div, Sao Paulo, Sp - Brazil

^[2] Butantan Inst, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Antimicrobial Chemotherapy; v. 73, n. 7, p. 1930-1934, JUL 2018.
• Citações Web of Science: 0

Resumo

Objectives: The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil. Methods: Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1. Results: Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies.20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies,14.8% did not persist upon established infection. The presence of transmitted drug resistance mutations was negatively correlated with the viral load and with CD4+T cell count decay. Conclusions: Transmitted drug resistance mutations representing small percentages of the viral population do not persist during infection because they are negatively selected in the first year after HIV-1 seroconversion. (AU)

• Processo FAPESP: 11/50238-8 - Estudos das quasispecies de hiv com uso do sequenciamento paralelo macico.
• Beneficiário: Ana Rachel Oliveira Léda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/12156-0 - Estudos das quasispecies do HIV com o uso de sequenciamento paralelo maciço
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Regular