| Full text | |
| Author(s): |
Leda, Ana Rachel
[1]
;
Hunter, James
[1]
;
Oliveira, Ursula Castro
[2]
;
Azevedo, Inacio Junqueira
[2]
;
Araripe Sucupira, Maria Cecilia
[1]
;
Diaz, Ricardo Sobhie
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Med, Infect Dis Div, Sao Paulo, Sp - Brazil
[2] Butantan Inst, Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of Antimicrobial Chemotherapy; v. 73, n. 7, p. 1930-1934, JUL 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Objectives: The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil. Methods: Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1. Results: Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies.20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies,14.8% did not persist upon established infection. The presence of transmitted drug resistance mutations was negatively correlated with the viral load and with CD4+T cell count decay. Conclusions: Transmitted drug resistance mutations representing small percentages of the viral population do not persist during infection because they are negatively selected in the first year after HIV-1 seroconversion. (AU) | |
| FAPESP's process: | 11/50238-8 - Estudos das quasispecies de hiv com uso do sequenciamento paralelo macico. |
| Grantee: | Ana Rachel Oliveira Léda |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 11/12156-0 - HIV quasispecies studies using ultra-deep sequencing |
| Grantee: | Ricardo Sobhie Diaz |
| Support Opportunities: | Regular Research Grants |