Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insights about minority HIV-1 strains in transmitted drug resistance mutation dynamics and disease progression

Full text
Author(s):
Leda, Ana Rachel [1] ; Hunter, James [1] ; Oliveira, Ursula Castro [2] ; Azevedo, Inacio Junqueira [2] ; Araripe Sucupira, Maria Cecilia [1] ; Diaz, Ricardo Sobhie [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Dept Med, Infect Dis Div, Sao Paulo, Sp - Brazil
[2] Butantan Inst, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Antimicrobial Chemotherapy; v. 73, n. 7, p. 1930-1934, JUL 2018.
Web of Science Citations: 0
Abstract

Objectives: The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil. Methods: Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1. Results: Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies.20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies,14.8% did not persist upon established infection. The presence of transmitted drug resistance mutations was negatively correlated with the viral load and with CD4+T cell count decay. Conclusions: Transmitted drug resistance mutations representing small percentages of the viral population do not persist during infection because they are negatively selected in the first year after HIV-1 seroconversion. (AU)

FAPESP's process: 11/12156-0 - HIV quasispecies studies using ultra-deep sequencing
Grantee:Ricardo Sobhie Diaz
Support type: Regular Research Grants