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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insights about minority HIV-1 strains in transmitted drug resistance mutation dynamics and disease progression

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Leda, Ana Rachel [1] ; Hunter, James [1] ; Oliveira, Ursula Castro [2] ; Azevedo, Inacio Junqueira [2] ; Araripe Sucupira, Maria Cecilia [1] ; Diaz, Ricardo Sobhie [1]
Total Authors: 6
[1] Univ Fed Sao Paulo, Dept Med, Infect Dis Div, Sao Paulo, Sp - Brazil
[2] Butantan Inst, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Antimicrobial Chemotherapy; v. 73, n. 7, p. 1930-1934, JUL 2018.
Web of Science Citations: 0

Objectives: The presence of minority transmitted drug resistance mutations was assessed using ultra-deep sequencing and correlated with disease progression among recently HIV-1-infected individuals from Brazil. Methods: Samples at baseline during recent infection and 1 year after the establishment of the infection were analysed. Viral RNA and proviral DNA from 25 individuals were subjected to ultra-deep sequencing of the reverse transcriptase and protease regions of HIV-1. Results: Viral strains carrying transmitted drug resistance mutations were detected in 9 out of the 25 patients, for all major antiretroviral classes, ranging from one to five mutations per patient. Ultra-deep sequencing detected strains with frequencies as low as 1.6% and only strains with frequencies.20% were detected by population plasma sequencing (three patients). Transmitted drug resistance strains with frequencies,14.8% did not persist upon established infection. The presence of transmitted drug resistance mutations was negatively correlated with the viral load and with CD4+T cell count decay. Conclusions: Transmitted drug resistance mutations representing small percentages of the viral population do not persist during infection because they are negatively selected in the first year after HIV-1 seroconversion. (AU)

FAPESP's process: 11/12156-0 - HIV quasispecies studies using ultra-deep sequencing
Grantee:Ricardo Sobhie Diaz
Support type: Regular Research Grants