Enzymatic Activity Prediction Using Time-Domain Nuclear Magnetic Resonance (TD-NMR) and Multivariate Analysis: A Case Study Using Cassava Roots

Autor(es)

Ferreira, Josilei da Silva ^[1] ; Moraes, Tiago Bueno ^[2] ; Colnago, Luiz Alberto ^[3] ; Verbi Pereira, Fabiola Manhas ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Inst Quim Araraquara, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[3] Embrapa Instrumentacao, BR-13560570 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPLIED MAGNETIC RESONANCE; v. 49, n. 7, p. 653-664, JUL 2018.
• Citações Web of Science: 0

Resumo

Time-domain nuclear magnetic resonance (NMR) has been widely used in food science. In this work, we demonstrate that the NMR decay obtained with the Carr-Purcell-Meiboom-Gill (CPMG) sequence can be used to estimate the peroxidase activity (PA) in cassava roots. This enzyme has been involved in post-harvest physiological deterioration (PPD), which limits the storage of fresh cassava to a few days. Cassava is a staple food for almost one billion people in tropical areas in Americas, Africa and Asia. A multivariate method using CPMG data and reference values of PA from a standard biochemical assay was built with 216 measurements for non-refrigerated and refrigerated samples of cassava roots. The figures of merit of the global partial least squares model using both types of roots showed a 0.06 mu mol min(-1) limit of detection (LOD) and a 0.2 mu mol min(-1) limit of quantification (LOQ) for PA, with 0.4 {[}intensity (a.u.)/(mu mol min(-1))] sensitivity and a standard error of cross-validation (SECV) of 0.7 mu mol min(-1). All of the results demonstrated that TD-NMR has the potential to predict PA in cassava roots that is indicative of the PPD problem. (AU)

• Processo FAPESP: 16/00779-6 - Obtenção direta de informações químicas de interesse comercial de matrizes analíticas sólidas diversificadas utilizando a espectroscopia de emissão com plasma induzido por laser (LIBS) e quimiometria
• Beneficiário: Hideko Yamanaka
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/12864-0 - Avaliação do efeito do campo magnético dos espectrômetro de RMN em reações químicas monitoradas in situ
• Beneficiário: Luiz Alberto Colnago
• Modalidade de apoio: Auxílio à Pesquisa - Regular