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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enzymatic Activity Prediction Using Time-Domain Nuclear Magnetic Resonance (TD-NMR) and Multivariate Analysis: A Case Study Using Cassava Roots

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Author(s):
Ferreira, Josilei da Silva [1] ; Moraes, Tiago Bueno [2] ; Colnago, Luiz Alberto [3] ; Verbi Pereira, Fabiola Manhas [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista UNESP, Inst Quim Araraquara, BR-14800060 Araraquara, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
[3] Embrapa Instrumentacao, BR-13560570 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: APPLIED MAGNETIC RESONANCE; v. 49, n. 7, p. 653-664, JUL 2018.
Web of Science Citations: 0
Abstract

Time-domain nuclear magnetic resonance (NMR) has been widely used in food science. In this work, we demonstrate that the NMR decay obtained with the Carr-Purcell-Meiboom-Gill (CPMG) sequence can be used to estimate the peroxidase activity (PA) in cassava roots. This enzyme has been involved in post-harvest physiological deterioration (PPD), which limits the storage of fresh cassava to a few days. Cassava is a staple food for almost one billion people in tropical areas in Americas, Africa and Asia. A multivariate method using CPMG data and reference values of PA from a standard biochemical assay was built with 216 measurements for non-refrigerated and refrigerated samples of cassava roots. The figures of merit of the global partial least squares model using both types of roots showed a 0.06 mu mol min(-1) limit of detection (LOD) and a 0.2 mu mol min(-1) limit of quantification (LOQ) for PA, with 0.4 {[}intensity (a.u.)/(mu mol min(-1))] sensitivity and a standard error of cross-validation (SECV) of 0.7 mu mol min(-1). All of the results demonstrated that TD-NMR has the potential to predict PA in cassava roots that is indicative of the PPD problem. (AU)

FAPESP's process: 16/00779-6 - Direct obtaining of chemical information with commercial purposes from several solid analytical matrices using laser induced breakdown spectroscopy (LIBS) and Chemometrics
Grantee:Hideko Yamanaka
Support Opportunities: Regular Research Grants
FAPESP's process: 17/12864-0 - Effect of NMR magnetic field in chemical reactions monitored NMR in situ
Grantee:Luiz Alberto Colnago
Support Opportunities: Regular Research Grants