Molecular detection of fungi of public health importance in wild animals from Southern Brazil

Autor(es):Mostrar menos -

Losnak, Debora O. ^[1] ; Rocha, Francielle R. ^[2] ; Almeida, Barbara S. ^[2] ; Batista, Keila Z. S. ^[3] ; Althoff, Sergio L. ^[4] ; Haupt, Josiane ; Ruiz, Luciana S. ^[2] ; Anversa, Lais ^[2] ; Lucheis, Simone B. ^[5] ; Paiz, Lais M. ^[6] ; Donalisio, Maria Rita ^[6] ; Richini Pereira, Virginia B. ^[2]

Afiliação do(s) autor(es)

^[1] Unesp Univ Estadual Paulista, Botucatu Med Sch, Dept Trop Dis & Imaging Diag, Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Ctr Reg Labs Bauru 2, Ctr Biomed Sci, Sao Paulo - Brazil

^[3] Univ Reg Blumenau, Lab Immunol, Dept Nat Sci, Blumenau, SC - Brazil

^[4] Univ Reg Blumenau, Lab Anim Biol, Dept Nat Sci, Blumenau, SC - Brazil

^[5] Paulista Agcy Agribusiness Technol, Bauru - Brazil

^[6] State Univ Campinas UNICAMP, Sch Med Sci, Dept Publ Hlth, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MYCOSES; v. 61, n. 7, p. 455-463, JUL 2018.
• Citações Web of Science: 0

Resumo

Some animals have an important relationship with fungal infections, and searching for pathogens in animal samples may be an opportunity for eco-epidemiological research. Since studies involving wildlife are generally restricted, using samples from road kills is an alternative. The aim of this study was to verify whether pathogenic fungi of public health importance occur in wildlife road kills from Santa Catarina State, Brazil. Organ samples (n=1063) from 297 animals were analysed according to Polymerase Chain Reaction (PCR) using universal primers to detect fungi in general and, subsequently, using primers specific to Paracoccidioides brasiliensis, Histoplasma capsulatum and Cryptococcus spp. There were 102 samples positive for fungal species. Eight samples were positive for P.brasiliensis, three samples were positive for Cryptococcus spp. and one sample had coinfection by these two fungi. No sample was positive for Histoplasma spp. according to the molecular detection. Genetic sequencing allowed the identification of Fungal sp. in 89 samples, Cryptococcus neoformans in two samples and Aspergillus penicillioides in three samples. This study shows the importance of wild animals in the epidemiology of fungal infections and assists in the mapping of pathogen occurrence in a region that was not previously evaluated. (AU)

• Processo FAPESP: 15/17519-4 - Epidemiologia e diversidade genética de micro-organismos e suas implicações em saúde pública
• Beneficiário: Virgínia Bodelão Richini Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular