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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular detection of fungi of public health importance in wild animals from Southern Brazil

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Author(s):
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Losnak, Debora O. [1] ; Rocha, Francielle R. [2] ; Almeida, Barbara S. [2] ; Batista, Keila Z. S. [3] ; Althoff, Sergio L. [4] ; Haupt, Josiane ; Ruiz, Luciana S. [2] ; Anversa, Lais [2] ; Lucheis, Simone B. [5] ; Paiz, Lais M. [6] ; Donalisio, Maria Rita [6] ; Richini Pereira, Virginia B. [2]
Total Authors: 12
Affiliation:
[1] Unesp Univ Estadual Paulista, Botucatu Med Sch, Dept Trop Dis & Imaging Diag, Sao Paulo - Brazil
[2] Adolfo Lutz Inst, Ctr Reg Labs Bauru 2, Ctr Biomed Sci, Sao Paulo - Brazil
[3] Univ Reg Blumenau, Lab Immunol, Dept Nat Sci, Blumenau, SC - Brazil
[4] Univ Reg Blumenau, Lab Anim Biol, Dept Nat Sci, Blumenau, SC - Brazil
[5] Paulista Agcy Agribusiness Technol, Bauru - Brazil
[6] State Univ Campinas UNICAMP, Sch Med Sci, Dept Publ Hlth, Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: MYCOSES; v. 61, n. 7, p. 455-463, JUL 2018.
Web of Science Citations: 0
Abstract

Some animals have an important relationship with fungal infections, and searching for pathogens in animal samples may be an opportunity for eco-epidemiological research. Since studies involving wildlife are generally restricted, using samples from road kills is an alternative. The aim of this study was to verify whether pathogenic fungi of public health importance occur in wildlife road kills from Santa Catarina State, Brazil. Organ samples (n=1063) from 297 animals were analysed according to Polymerase Chain Reaction (PCR) using universal primers to detect fungi in general and, subsequently, using primers specific to Paracoccidioides brasiliensis, Histoplasma capsulatum and Cryptococcus spp. There were 102 samples positive for fungal species. Eight samples were positive for P.brasiliensis, three samples were positive for Cryptococcus spp. and one sample had coinfection by these two fungi. No sample was positive for Histoplasma spp. according to the molecular detection. Genetic sequencing allowed the identification of Fungal sp. in 89 samples, Cryptococcus neoformans in two samples and Aspergillus penicillioides in three samples. This study shows the importance of wild animals in the epidemiology of fungal infections and assists in the mapping of pathogen occurrence in a region that was not previously evaluated. (AU)

FAPESP's process: 15/17519-4 - Epidemiology and genetic diversity of microorganisms and its implications to public health
Grantee:Virgínia Bodelão Richini Pereira
Support Opportunities: Regular Research Grants