Investigation and phylogenetic characterization of Coronavirus in biota of wild and synanthropic birds from the southern and southeastern Brazil

• Grant number: 13/03922-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2013
• Effective date (End): April 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Clarice Weis Arns
• Grantee: Ricardo Durães de Carvalho
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The larg diversity of native species, synanthropic or not, and migratory birds, potentially hosts a wide variety of bacterial, fungal and viral agents, whose identity is virtually unknown and may be targets for deep scientific studies. Besides representing a wealth of biodiversity, scientific exploration and commercial potential, these agents may pose potential health risks. Thus, this study focuses on identifying and characterizing genetically coronaviruses circulating in wild birds or native synanthropic and migratory seabirds in the State of São Paulo. Samples of tracheal swabs and cloacal swabs will be collected from various species of native wild birds and / or synanthropic sorting centers in the state; samples of species of seabirds will be collected in coastal sorting centers. The samples will be examined by the technique of "Real Time RT-PCR Multiprobe" for the presence of coronavirus belonging to 3 genera (pancoronavírus). The identified agents will be submitted to the sequencing of genes S, N and Pol followed by phylogenetic analysis. In addition to being a representative database of the State's biodiversity, the study of these viral agents will be extremely useful for the elucidation of works involving molecular epidemiology and ecology.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DURAES-CARVALHO, RICARDO; CASERTA, LEONARDO C.; BARNABE, ANA C. S.; MARTINI, MATHEUS C.; SIMAS, PAULO V. M.; SANTOS, MARCIA M. B.; SALEMI, MARCO; ARNS, CLARICE W.. Phylogenetic and phylogeographic mapping of the avian coronavirus spike protein-encoding gene in wild and synanthropic birds. VIRUS RESEARCH, v. 201, p. 101-112, APR 2 2015. (13/03922-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARVALHO, Ricardo Durães de. Investigation and phylogenetic characterization of Coronavirus in biota of wild and synanthropic birds from Southern and Southeastern Brazil. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.