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Investigation and phylogenetic characterization of Coronavirus in biota of wild and synanthropic birds from Southern and Southeastern Brazil

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Author(s):
Ricardo Durães de Carvalho
Total Authors: 1
Document type: Doctoral Thesis
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
Defense date:
Examining board members:
Clarice Weis Arns; Claudio Chrysostomo Werneck; Marcelo Lancellotti; Luiz Carlos Júnior Alcântara; Maria Angela Orsi
Advisor: Clarice Weis Arns; Márcia Mercês Aparecida Bianchi dos Santos
Abstract

The evolution and population dynamics of Coronaviruses (CoVs) still remain underexplored. In the present study, phylogenetic and phylogeographic analyseswere conducted to investigate the evolutionary dynamics of CoV detected in wild and synanthropic birds. A total of 500 samples, including tracheal and cloacal swabs collected from 312 wild birds belonging to 42species, were analysed by RT-qPCR. A total of 65 samples from 23bird species were positive for Avian Coronaviruses (AvCoVs).Three hundred and two samples were screened for the Pan-Coronavirus (Pan-CoV) through the nPCR, 17 (5.6%) were positive, being that 11 were detected in different species. AvCoVs phylogenetic analyses revealed that the DNA sequences from samples collected in Brazil did not cluster with any of the AvCoV S1 gene sequences deposited in the GenBank database. Bayesian framework analysis estimated an AvCoV strain from Sweden (1999) as the most recent common ancestor of the AvCoVs detected in this study. Furthermore, Bayesian Skyline Plot (BSP) analysis inferred an increase in the AvCoV dynamic demographic population in different wild and synanthropic bird species. Phylogenetic analysis of the Pan-CoV showed that most of the samples clustered with the Murine Hepatitis Virus A59 strain (MHV A59) belong to the BetaCoV group. Besides, one of our samples [CoV detected in Amazona vinacea (parrot-breasted-purple)] clustered with a CoV isolated from pigs, PCoV HKU15, belonging to the DeltaCoV genus, still not reported in South America. Our findings suggest that birds may be potential new hosts responsible for spreading of different CoVs for different species of animals (AU)

FAPESP's process: 13/03922-6 - Investigation and phylogenetic characterization of Coronavirus in biota of wild and synanthropic birds from the southern and southeastern Brazil
Grantee:Ricardo Durães de Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate