Crude leaf extracts of Piperaceae species downmodulate inflammatory responses by human monocytes

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Finato, Angela Carolina ^[1] ; Frage-Silva, Thais Fernanda ^{[1, 2]} ; Carvalho Prati, Amanda Uliana ^[3] ; de Souza Junior, Amauri Alves ^[3] ; Mazzeu, Bruna Fonseca ^[3] ; Felippe, Lidiane Gaspareto ^[3] ; Pinto, Rute Alves ^[3] ; Golim, Marjorie de Assis ^[4] ; Parreira Arruda, Maria Sueli ^[1] ; Furlan, Maysa ^[3] ; Venturini, James ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Fac Ciencias, Bauru, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Inst Biociencias, Bauru, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Quim, Araraquara, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Fac Med, Botucatu, SP - Brazil

^[5] Univ Fed Mato Grosso Sul UFMS, Fac Med, Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 6 JUN 20 2018.
• Citações Web of Science: 2

Resumo

In this study, we aimed to evaluate the immunomodulatory effects of crude leaf extracts from Piper gaudichaudianum Kunth, P. arboreum Aub., P. umbellata L., P. fuligineum Kunth, and Peperomia obtusifolia A. Dietr. on an in vitro model of inflammatory response. The crude extracts were previously obtained by maceration of the leaves. The half-maximal inhibitory concentration was determined by the MTT assay using human peripheral blood mononuclear cells. Human monocytes were simultaneously challenged with each crude extract and lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, to induce a strong inflammatory response. After 24 h of incubation, cell-free supernatants were used for evaluating the mediators involved in inflammation: H2O2, TNF-alpha, IL-8, IL-6, IL-1 beta, IL-10, IL-12, FGF-b, and TGF-beta 1. We also compared the results with the effects of ketoprofen, a well-known anti-inflammatory drug. The P. gaudichaudianum crude extract downmodulated the production of H2O2, IL-1 beta, IL-6, IL-8, and TGF-beta 1 by LPS-stimulated monocytes; P. arboreum, IL-1 beta, IL-6, IL-8, and TNF-alpha; P. umbellata and P. fuligineum, H2O2, IL-1 beta, IL-6, IL-8, IL-10, and TNF-alpha; and P. obtusifolia, H2O2, IL-6, IL-8, IL-10, and TNF-alpha. In general, the crude leaf extracts amplified the anti-inflammatory response when compared with ketoprofen, particularly reducing the production of IL-8, a mediator involved in neutrophil recruitment during tissue damage. Thus, the crude leaf extracts of P. gaudichaudianum, P. arboreum, P. umbellata, P. fuligineum, and Peperomia obtusifolia elicited an anti-inflammatory response against LPS-challenged monocytes. These findings show the anti-inflammatory properties of these crude leaf extracts and offer new perspectives for their use in the treatment of inflammatory diseases. (AU)

• Processo FAPESP: 16/17627-4 - Potencial imunomodulador de substâncias bioativas vegetais na ativação do inflamassoma e na polarização de macrófagos M1 e M2
• Beneficiário: Maria Sueli Parreira de Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Regular