Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test

Autor(es)

Yoshida, Edson Hideaki ^[1] ; Tribuiani, Natalia ^[1] ; Sabadim, Giovana ^[1] ; Neto Moreno, Debora Antunes ^[1] ; Varanda, Eliana Aparecida ^[2] ; Oshima-Franco, Yoko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sorocaba UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Pharmaceut Sci Fac Araraquara, Rodovia Araraquara Jau, Km 1, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ADVANCED PHARMACEUTICAL BULLETIN; v. 6, n. 3, p. 443-447, SEP 2016.
• Citações Web of Science: 1

Resumo

Purpose: Betulin is a pentacyclic triterpene found in the outer barks of innumerous plants. This secondary metabolite is easily isolated from plants with the major interest in converting it to betulinic acid, which pharmacological properties were much more exploited than betulin. But, investments in the own betulin have been grown since no chemical step is necessary. In this study we focused the precursor betulin in order to evaluate its mutagenicity by Salmonella/microsome assay (Ames test). Methods: The Ames test was carried out using a commercial betulin exposed to Salmonella typhimurium strains TA98, TA100, TA102, and TA97a, in experiments with (+S9) and without (-S9) metabolic activation. Results: Betulin was unable to increase the number of revertants (+S9 and -S9 metabolic activation) showing the absence of any mutagenic effect by Ames test. Conclusion: This study allowed attribute safety to betulin being important for exploiting its pharmacological uses. (AU)

• Processo FAPESP: 08/11005-5 - Dipteryx alata Vogel: estudos da ação antiofídica e de toxicidade
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 04/09705-8 - Investigacao farmacologica da atividade antipeconhenta de fracoes obtidas de extratos de casearia sylvestris sw.
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/08271-0 - IDENTIFICAÇÃO E FARMACODINÂMICA DE FRAÇÕES NEUROFACILITADORAS DA Casearia sylvestris SW. NA JUNÇÃO NEUROMUSCULAR
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52643-4 - Estudo da capacidade neutralizante do extrato hidroalcoolico de camellia sinensis (cha verde) contra os efeitos neurotoxico e miotoxico da bothropstoxina-i (bothrops jararacussu).
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/53883-6 - Dipteryx alata vogel: estudo da acao antiofidica e da toxicidade.
• Beneficiário: Yoko Oshima Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular