Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test

Author(s)

Yoshida, Edson Hideaki ^[1] ; Tribuiani, Natalia ^[1] ; Sabadim, Giovana ^[1] ; Neto Moreno, Debora Antunes ^[1] ; Varanda, Eliana Aparecida ^[2] ; Oshima-Franco, Yoko ^[1]

Affiliation

^[1] Univ Sorocaba UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Pharmaceut Sci Fac Araraquara, Rodovia Araraquara Jau, Km 1, BR-14801902 Araraquara, SP - Brazil

• Document type: Journal article
• Source: ADVANCED PHARMACEUTICAL BULLETIN; v. 6, n. 3, p. 443-447, SEP 2016.
• Web of Science Citations: 1

Abstract

Purpose: Betulin is a pentacyclic triterpene found in the outer barks of innumerous plants. This secondary metabolite is easily isolated from plants with the major interest in converting it to betulinic acid, which pharmacological properties were much more exploited than betulin. But, investments in the own betulin have been grown since no chemical step is necessary. In this study we focused the precursor betulin in order to evaluate its mutagenicity by Salmonella/microsome assay (Ames test). Methods: The Ames test was carried out using a commercial betulin exposed to Salmonella typhimurium strains TA98, TA100, TA102, and TA97a, in experiments with (+S9) and without (-S9) metabolic activation. Results: Betulin was unable to increase the number of revertants (+S9 and -S9 metabolic activation) showing the absence of any mutagenic effect by Ames test. Conclusion: This study allowed attribute safety to betulin being important for exploiting its pharmacological uses. (AU)

• FAPESP's process: 08/11005-5 - Dipteryx alata Vogel: antiophidian action and toxicity
• Grantee: Yoko Oshima Franco
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants