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Mutagenicity and evaluation of chemical composition of atmospheric particulate mater from Limeira, Stolckholm and Kyoto

Grant number: 15/23364-3
Support Opportunities:Regular Research Grants
Duration: March 01, 2016 - February 28, 2018
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal Investigator:Fábio Kummrow
Grantee:Fábio Kummrow
Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
Associated researchers: Bianca de Souza Maselli ; Gisela de Aragão Umbuzeiro


The atmospheric particulate matter (PM) is associated with multiple injuries and diseases, being recently classified as carcinogenic to humans by IARC and recognized as a mutagen to germ cells. Although its toxicity, including genotoxic effects, is admittedly linked to the size of the particles, the contribution of their chemical composition for these effects are not well established. It is known that these effects are caused either by toxicants emitted directly into the environment, and by those formed by atmospheric reactions, which are huge influenced by regional and climatic characteristics. In order to identify mutagens in PM, approaches that combined mutagenicity tests employing bacteria with high throughput chemical analysis, for example, mass spectrometry cyclotron resonance of ions and Fourier transform (FT-ICR MS) has been internationally recommended. The objective of this multicenter study is to investigate the influence of atmospheric and climatic conditions of Limeira (Brazil), Stockholm (Sweden) and Kyoto (Japan) in the chemical composition and mutagenic activity of PM collected during the winter in these cities. The Salmonella/microsome test with different diagnostic strains will be used to evaluate organic extracts of PM samples. The data will be used to direct the mutagens analytical identification step. Samples will be collected and processed using the same protocols allowing direct comparison of results. The sample preparation and fractionation of the extracts will be held in Sweden. Chemical analysis will be carried out in Sweden, Japan and Brazil and the mutagenicity profile in Brazil. The intent of this project is to consolidate the use of diagnostic test strains of Salmonella/microsome, especially YG5185 never used for this purpose, the characterization of mutagenicity PM and driving of chemical identification analysis. If successful, the FT-ICR MS technique could prove very important in helping to identify chemical mutagens. Weather conditions and the transport of air masses that influenced the formation of secondary pollutants in each collection site during the study period will be identified and the data used to suggest possible sources and processes of formation of mutagenic compounds and mutagenic activity profile identified. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MASELLI, BIANCA S.; GIRON, MACELLE C. G.; LIM, HWANMI; BERGVALL, CHRISTOFFER; WESTERHOLM, ROGER; DREIJ, KRISTIAN; WATANABE, TETSUSHI; CARDOSO, ARNALDO A.; UMBUZEIRO, GISELA A.; KUMMROW, FABIO. Comparative mutagenic activity of atmospheric particulate matter from limeira, stockholm, and kyoto. Environmental and Molecular Mutagenesis, v. 60, n. 7, p. 607-616, . (15/23364-3)
MASELLI, BIANCA S.; CUNHA, VIRGINIA; LIM, HWANMI; BERGVALL, CHRISTOFFER; WESTERHOLM, ROGER; DREIJ, KRISTIAN; WATANABE, TETSUSHI; CARDOSO, ARNALDO A.; POZZA, SIMONE A.; UMBUZEIRO, GISELA A.; et al. Similar polycyclic aromatic hydrocarbon and genotoxicity profiles of atmospheric particulate matter from cities on three different continents. Environmental and Molecular Mutagenesis, v. 61, n. 5, . (15/23364-3)

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