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Virtual effect directed analysis for mutagenicity characterization of relevant environmental samples

Grant number: 15/24758-5
Support type:Regular Research Grants
Duration: August 01, 2016 - July 31, 2018
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal researcher:Gisela de Aragão Umbuzeiro
Grantee:Gisela de Aragão Umbuzeiro
Home Institution: Faculdade de Tecnologia (FT). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

Effect Directed Analysis (EDA) have been shown the best way to diclose new priority pollutants. Bioassays, like the Salmonella/microsome assay (Ames test), have been used susccessfuly in EDA studies, but with limitations when the samples are not potent enough to be fractionated and re-tested. With the advance of the chemical techniques in the non-target analysis, the ability to identify potential chemical pollutants in water increased substantially. As a consequence new ways of doing EDA has emerged. Virtual EDA (vEDA is one of those approaches. The basic concept of lower-tier virtual EDA (vEDA) is to combine information derived from mass spectral analysis and a bioassay using multivariate statistics to isolate compounds or peaks, in case of non-target analysis that co-vary with the biological responses. It has the advantage of avoiding the first fractionation step and reducing the number of tests. It was never used in mutagenicity evaluation. The objective of this study is to apply a virtual EDA using the results of the diagnostic strains of the Salmonella microsome assay. We will analyze water samples from the Danube river as a model and then apply to the same approach to Santos outfall samples. Water phase and suspended particulate matter will be analyzed and the data compared. The proposed project will be part of a 5 year- collaborative solution-oriented project already approved by the European Commission - EU Work Programme 2013 named "SOLUTIONS for present and future emerging pollutants in land and water resources management". We intend to compare the mutagenic and chemical profile of organic extracts of water and suspended particulate matter samples using the tools as already developed in the project, supporting the selection of priority pollutants in an integrated risk based approach. During the last two years, the participation of our group in the european Project was very productive and the approval of this project will build a even stronger collaboration with the european scientists involved in SOLUTIONS allowing the development of new tools to deal with our complex environmental problems. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VACCHI, I, FRANCINE; DOS SANTOS, AMANDA; ARTAL, MARIANA C.; MAGALHAES, GABRIEL R.; DE SOUZA VENDEMIATTI, JOSIANE A.; UMBUZEIRO, GISELA DE ARAGAO. Parhyale hawaiensis as a promising alternative organism for monitoring acute toxicity of sediments under the influence of submarine outfalls. Marine Pollution Bulletin, v. 149, DEC 2019. Web of Science Citations: 0.
ALTENBURGER, ROLF; BRACK, WERNER; BURGESS, ROBERT M.; BUSCH, WIBKE; ESCHER, BEATE I.; FOCKS, ANDREAS; HEWITT, L. MARK; JACOBSEN, BO N.; LOPEZ DE ALDA, MIREN; AIT-AISSA, SELIM; BACKHAUS, THOMAS; GINEBREDA, ANTONI; HILSCHEROVA, KLARA; HOLLENDER, JULIANE; HOLLERT, HENNER; NEALE, PETA A.; SCHULZE, TOBIAS; SCHYMANSKI, EMMA L.; TEODOROVIC, IVANA; TINDALL, ANDREW J.; UMBUZEIRO, GISELA DE ARAGAO; VRANA, BRANISLAV; ZONJA, BOZO; KRAUSS, MARTIN. Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures. ENVIRONMENTAL SCIENCES EUROPE, v. 31, FEB 19 2019. Web of Science Citations: 17.
BRACK, WERNER; AISSA, SELIM AIT; BACKHAUS, THOMAS; DULIO, VALERIA; ESCHER, BEATE I.; FAUST, MICHAEL; HILSCHEROVA, KLARA; HOLLENDER, JULIANE; HOLLERT, HENNER; MUELLER, CHRISTIN; MUNTHE, JOHN; POSTHUMA, LEO; SEILER, THOMAS-BENJAMIN; SLOBODNIK, JAROSLAV; TEODOROVIC, IVANA; TINDALL, ANDREW J.; UMBUZEIRO, GISELA DE ARAGAO; ZHANG, XIAOWEI; ALTENBURGER, ROLF. Effect-based methods are key. The European Collaborative Project SOLUTIONS recommends integrating effect-based methods for diagnosis and monitoring of water quality. ENVIRONMENTAL SCIENCES EUROPE, v. 31, FEB 8 2019. Web of Science Citations: 24.
ZWARG, JOSE RICARDO R. M.; MORALES, DANIEL A.; MASELLI, BIANCA S.; BRACK, WERNER; UMBUZEIRO, GISELA A. Miniaturization of the microsuspension Salmonella/microsome assay in agar microplates. Environmental and Molecular Mutagenesis, v. 59, n. 6, p. 488-501, JUL 2018. Web of Science Citations: 4.
ALTENBURGER, ROLF; SCHOLZE, MARTIN; BUSCH, WIBKE; ESCHER, BEATE I.; JAKOBS, GIANINA; KRAUSS, MARTIN; KRUEGER, JANET; NEALE, PETA A.; AIT-AISSA, SELIM; ALMEIDA, ANA CATARINA; SEILER, THOMAS-BENJAMIN; BRION, FRANCOIS; HILSCHEROVA, KLARA; HOLLERT, HENNER; NOVAK, JIRI; SCHLICHTING, RITA; SERRA, HELENE; SHAO, YING; TINDALL, ANDREW; TOLEFSEN, KNUT-ERIK; UMBUZEIRO, GISELA; WILLIAMS, TIM D.; KORTENKAMP, ANDREAS. Mixture effects in samples of multiple contaminants - An inter-laboratory study with manifold bioassays. Environment International, v. 114, p. 95-106, MAY 2018. Web of Science Citations: 22.
NEALE, PETA A.; ALTENBURGER, ROLF; AIT-AISSA, SELIM; BRION, FRANCOIS; BUSCH, WIBKE; UMBUZEIRO, GISELA DE ARAGAO; DENISON, MICHAEL S.; DU PASQUIER, DAVID; HILSCHEROVA, KLARA; HOLLERT, HENNER; MORALES, DANIEL A.; NOVAK, JIRI; SCHLICHTING, RITA; SEILER, THOMAS-BENJAMIN; SERRA, HELENE; SHAO, YING; TINDALL, ANDREW J.; TOLLEFSEN, KNUT ERIK; WILLIAMS, TIMOTHY D.; ESCHER, BEATE I. Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their Contribution to effects in surface water. WATER RESEARCH, v. 123, p. 734-750, OCT 15 2017. Web of Science Citations: 59.

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