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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rhizoctonia solani fucomannogalactan: Chemical characterization and antiproliferative activity

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Autor(es):
Alexandre, Samara M. A. [1] ; Corradi da Silva, Maria L. [1] ; Vasconcelos, Ana F. D. [1] ; Exposti, Diego T. D. [1] ; Tischer, Cesar A. [2] ; Prieto, Alicia [3] ; Diaz, Dolores [3] ; Kaneno, Ramon [4]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Dept Chem & Biochem, Sch Sci & Technol, BR-19060900 Presidente Prudente - Brazil
[2] Univ Estadual Londrina, Dept Biochem & Biotechnol, BR-86057970 Londrina - Brazil
[3] CSIC, Biol Res Ctr CIB, Madrid 28006 - Spain
[4] Sao Paulo State Univ UNESP, Dept Microbiol & Immunol, Inst Biosci, BR-18618689 Botucatu, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 115, p. 106-113, AUG 2018.
Citações Web of Science: 1
Resumo

A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (M-w/M-n similar to 1.1), displaying an average molecular weight of 15.4 x 10(3) Da. Its chemical structure was determined by methylation analysis (GC/MS) and spectroscopy (FTIR, 1D and 2D NMR). The polysaccharide had a branched alpha-1,6-linked Galp backbone with 66% linear residues, a number of which were at O-3 methylated. Side chains (34%) were always linked at O-2 positions of the main chain and consisted of single, non reducing ends of alpha-D-Manp (6%) and alpha-L-Fucp (28%). Analysis of its biological activity showed that the highly purified fucomannogalactan from R. solani inhibited the proliferation of colon cancer cells in vitro, but that it did not have the same activity against lung cancer cells. (C) 2018 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/02601-1 - Prospecção de polissacarídeos fúngicos para aplicação biomédica: utilização de ressonância magnética nuclear e espectrometria de massa para caracterização estrutural
Beneficiário:Maria de Lourdes Corradi Custodio da Silva
Linha de fomento: Bolsas no Exterior - Pesquisa