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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rhizoctonia solani fucomannogalactan: Chemical characterization and antiproliferative activity

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Author(s):
Alexandre, Samara M. A. [1] ; Corradi da Silva, Maria L. [1] ; Vasconcelos, Ana F. D. [1] ; Exposti, Diego T. D. [1] ; Tischer, Cesar A. [2] ; Prieto, Alicia [3] ; Diaz, Dolores [3] ; Kaneno, Ramon [4]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Dept Chem & Biochem, Sch Sci & Technol, BR-19060900 Presidente Prudente - Brazil
[2] Univ Estadual Londrina, Dept Biochem & Biotechnol, BR-86057970 Londrina - Brazil
[3] CSIC, Biol Res Ctr CIB, Madrid 28006 - Spain
[4] Sao Paulo State Univ UNESP, Dept Microbiol & Immunol, Inst Biosci, BR-18618689 Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 115, p. 106-113, AUG 2018.
Web of Science Citations: 1
Abstract

A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (M-w/M-n similar to 1.1), displaying an average molecular weight of 15.4 x 10(3) Da. Its chemical structure was determined by methylation analysis (GC/MS) and spectroscopy (FTIR, 1D and 2D NMR). The polysaccharide had a branched alpha-1,6-linked Galp backbone with 66% linear residues, a number of which were at O-3 methylated. Side chains (34%) were always linked at O-2 positions of the main chain and consisted of single, non reducing ends of alpha-D-Manp (6%) and alpha-L-Fucp (28%). Analysis of its biological activity showed that the highly purified fucomannogalactan from R. solani inhibited the proliferation of colon cancer cells in vitro, but that it did not have the same activity against lung cancer cells. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/02601-1 - Fungi polysaccharides prospecting for medical application: structural characterization by nuclear magnetic resonance and mass spectrometry
Grantee:Maria de Lourdes Corradi Custodio da Silva
Support Opportunities: Scholarships abroad - Research