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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enzymatic production of cellulose nanofibers and sugars in a stirred-tank reactor: determination of impeller speed, power consumption, and rheological behavior

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Autor(es):
Bondancia, Thalita J. [1, 2] ; Correa, Luciano J. [3] ; Cruz, Antonio J. G. [1] ; Badino, Alberto C. [1] ; Mattoso, Luiz Henrique C. [2] ; Marconcini, Jose Manoel [2] ; Farinas, Cristiane S. [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, Rua 15 Novembro 1452, BR-13561206 Sao Carlos, SP - Brazil
[3] Univ Fed Lavras, Dept Engn, BR-3720000 Lavras, MG - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Cellulose; v. 25, n. 8, p. 4499-4511, AUG 2018.
Citações Web of Science: 2
Resumo

An integrated biorefinery process is proposed here for the enzymatic production of cellulose nanofiber (CNF) and sugars in a stirred-tank reactor using eucalyptus cellulose pulp as feedstock. Process engineering variables required for scale-up such as impeller speed, power consumption, and rheological behavior were determined under different experimental conditions of solids loading (10 and 15% w/v) and enzyme dosage (5 and 10 mg/g). Based on the mixing time, an impeller speed rotation of 470 rpm was selected for provision of adequate homogenization of the medium. Total energy consumption ranged from 161 to 207 W h and showed that significantly lower power consumption could be achieved using 10 mg/g enzyme loading with 10% w/v solids. Evaluation of rheological behavior showed that transition to a turbulent flow regime during the enzymatic hydrolysis reaction resulted in a constant power number ranging from 2.06 to 2.51, which was also lower for 10 mg/g enzyme loading with 10% w/v solids. Integrated analysis of glucose released and CNF generated after enzymatic hydrolysis showed that glucose values varied from 42.0 to 90.6 g/L, corresponding to cellulose conversion ranging from 57.2 to 76.4%. These values are suitable for the microbial fermentation of sugars into biofuels, while leaving a useful amount of residual nanomaterial. The residual solids of the enzymatic reactions presented the characteristics of CNF, as shown by X-ray diffraction (XRD) analyses, with crystallinity index (CI) values of 72-81%, as well as by morphological analysis using field emission scanning electron microscopy (FEG-SEM), which revealed diameters in the range 18-31 nm, making this nanomaterial suitable for use in a wide range of industrial applications. The findings indicated the potential of using conventional stirred-tank reactors for enzymatic hydrolysis for the integrated production of CNF and glucose, hence contributing to the implementation of future large-scale biorefineries. {[}GRAPHICS] (AU)

Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
Beneficiário:Roberto de Campos Giordano
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático