Fruit-localized phytochromes regulate plastid biogenesis, starch synthesis, and carotenoid metabolism in tomato

Autor(es)

Bianchetti, Ricardo Ernesto ^[1] ; Lira, Bruno Silvestre ^[1] ; Monteiro, Scarlet Santos ^[1] ; Demarco, Diego ^[1] ; Purgatto, Eduardo ^[2] ; Rothan, Christophe ^[3] ; Rossi, Magdalena ^[1] ; Freschi, Luciano ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil

^[3] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, F-33140 Villenave Dornon - France

• Tipo de documento: Artigo Científico
• Fonte: Journal of Experimental Botany; v. 69, n. 15, p. 3573-3586, JUL 10 2018.
• Citações Web of Science: 11

Resumo

Light signaling has long been reported to influence fruit biology, although the regulatory impact of fruit-localized photoreceptors on fruit development and metabolism remains unclear. Studies performed in phytochrome (PHY)deficient tomato (Solanum lycopersicum) mutants suggest that SlPHYA, SlPHYB2, and to a lesser extent SlPHYB1 influence fruit development and ripening. By employing fruit-specific RNAi-mediated silencing of SlPHY genes, we demonstrated that fruit-localized SlPHYA and SlPHYB2 play contrasting roles in regulating plastid biogenesis and maturation in tomato. Our data revealed that fruit-localized SlPHYA, rather than SlPHYB1 or SlPHYB2, positively influences tomato plastid differentiation and division machinery via changes in both light and cytokinin signaling-related gene expression. Fruit-localized SlPHYA and SlPHYB2 were also shown to modulate sugar metabolism in early developing fruits via overlapping, yet distinct, mechanisms involving the co-ordinated transcriptional regulation of genes related to sink strength and starch biosynthesis. Fruit-specific SlPHY silencing also drastically altered the transcriptional profile of genes encoding light-repressor proteins and carotenoid-biosynthesis regulators, leading to reduced carotenoid biosynthesis during fruit ripening. Together, our data reveal the existence of an intricate PHY-hormonal interplay during fruit development and ripening, and provide conclusive evidence on the regulation of tomato quality by fruit-localized phytochromes. (AU)

• Processo FAPESP: 13/18056-2 - Interação entre a sinalização luminosa, hormonal e do óxido nítrico durante a biogênese e diferenciação plastidial e acúmulo de compostos nutracêuticos em frutos de tomateiro
• Beneficiário: Luciano Freschi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01128-9 - Controle luminoso e hormonal da qualidade nutricional em Solanum lycopersicum
• Beneficiário: Maria Magdalena Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático