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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fruit-localized phytochromes regulate plastid biogenesis, starch synthesis, and carotenoid metabolism in tomato

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Author(s):
Bianchetti, Ricardo Ernesto [1] ; Lira, Bruno Silvestre [1] ; Monteiro, Scarlet Santos [1] ; Demarco, Diego [1] ; Purgatto, Eduardo [2] ; Rothan, Christophe [3] ; Rossi, Magdalena [1] ; Freschi, Luciano [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo - Brazil
[3] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, F-33140 Villenave Dornon - France
Total Affiliations: 3
Document type: Journal article
Source: Journal of Experimental Botany; v. 69, n. 15, p. 3573-3586, JUL 10 2018.
Web of Science Citations: 11
Abstract

Light signaling has long been reported to influence fruit biology, although the regulatory impact of fruit-localized photoreceptors on fruit development and metabolism remains unclear. Studies performed in phytochrome (PHY)deficient tomato (Solanum lycopersicum) mutants suggest that SlPHYA, SlPHYB2, and to a lesser extent SlPHYB1 influence fruit development and ripening. By employing fruit-specific RNAi-mediated silencing of SlPHY genes, we demonstrated that fruit-localized SlPHYA and SlPHYB2 play contrasting roles in regulating plastid biogenesis and maturation in tomato. Our data revealed that fruit-localized SlPHYA, rather than SlPHYB1 or SlPHYB2, positively influences tomato plastid differentiation and division machinery via changes in both light and cytokinin signaling-related gene expression. Fruit-localized SlPHYA and SlPHYB2 were also shown to modulate sugar metabolism in early developing fruits via overlapping, yet distinct, mechanisms involving the co-ordinated transcriptional regulation of genes related to sink strength and starch biosynthesis. Fruit-specific SlPHY silencing also drastically altered the transcriptional profile of genes encoding light-repressor proteins and carotenoid-biosynthesis regulators, leading to reduced carotenoid biosynthesis during fruit ripening. Together, our data reveal the existence of an intricate PHY-hormonal interplay during fruit development and ripening, and provide conclusive evidence on the regulation of tomato quality by fruit-localized phytochromes. (AU)

FAPESP's process: 13/18056-2 - Interaction between light, hormonal and nitric oxide signaling during plastidial biogenesis and differentiation and nutraceutical compounds accumulation in tomato fruits
Grantee:Luciano Freschi
Support Opportunities: Regular Research Grants
FAPESP's process: 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum
Grantee:Maria Magdalena Rossi
Support Opportunities: Research Projects - Thematic Grants