Evaluation of NKX3.1 and C-MYC expression in canine prostatic cancer

Autor(es)

Fonseca-Alves, Carlos Eduardo ^[1] ; Kobayashi, Priscila Emiko ^[1] ; Laufer-Amorim, Renee ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 118, p. 365-370, JUN 2018.
• Citações Web of Science: 6

Resumo

NKX3.1/C-MYC cross-regulation has been reported in the normal human prostate, and loss of NKX3.1 and gain of C-MYC seem to be important events in prostate cancer development and progression. The dog can be an interesting model for human prostatic disease, and yet only one previous research study has shown deregulation of NKX3.1 and MYC in the canine prostate. To address the expression of NKX3.1 and C-MYC in different canine prostatic lesions, this study verified the gene and protein expression of NKX3.1 and C-MYC in normal canine prostatic tissues. We identified a 26 kDa band that corresponded to the NKX3.1 protein, while C-MYC showed a 50 kDa band on Western blotting analysis of all prostatic tissues. We observed that NKX3.1 protein and transcript were down-regulated in prostate cancer (PC) samples compared with non-neoplastic samples. We also observed that C-MYC protein was overexpressed in PC samples compared with normal (P=.001) and proliferative inflammatory atrophy (PIA) samples (P=.003). We found a positive correlation between NKX3.1 and C-MYC protein expression in normal and PIA samples. Interestingly, a negative correlation (NKX3.1 downregulation and MYC overexpression) was observed between NKX3.1 and MYC transcripts in PC. Thus, samples with higher C-MYC expression also exhibited higher NKX3.1 expression, which indicates the regulation of C-MYC by NKX3.1 protein. As in humans, these two genes and proteins were found to be related to canine prostate cancer. However, in contrast from what is observed in humans, in canine PC samples, the downregulation of NKX3.1 cannot be explained by DNA hypermethylation. (AU)

• Processo FAPESP: 12/16068-0 - Avaliação epigenética dos genes NKX3.1 e CDH1 e expressão do c-myc, NKX3.1 e caderina-E por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Renee Laufer Amorim
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/18426-1 - Avaliação epigenética dos genes NKX3.1 e CDH1 e expressão do C-MYC, NKX3.1 e caderina-E por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Carlos Eduardo Fonseca Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/13774-6 - Expressão do C-MYC e NKX3.1 por Imunoistoquímica em Microarranjo de Tecido (TMA) de Lesões Pré-neoplásicas e Neoplásicas na Próstata de Cães
• Beneficiário: Carlos Eduardo Fonseca Alves
• Modalidade de apoio: Bolsas no Brasil - Mestrado