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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of NKX3.1 and C-MYC expression in canine prostatic cancer

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Author(s):
Fonseca-Alves, Carlos Eduardo [1] ; Kobayashi, Priscila Emiko [1] ; Laufer-Amorim, Renee [1]
Total Authors: 3
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Research in Veterinary Science; v. 118, p. 365-370, JUN 2018.
Web of Science Citations: 6
Abstract

NKX3.1/C-MYC cross-regulation has been reported in the normal human prostate, and loss of NKX3.1 and gain of C-MYC seem to be important events in prostate cancer development and progression. The dog can be an interesting model for human prostatic disease, and yet only one previous research study has shown deregulation of NKX3.1 and MYC in the canine prostate. To address the expression of NKX3.1 and C-MYC in different canine prostatic lesions, this study verified the gene and protein expression of NKX3.1 and C-MYC in normal canine prostatic tissues. We identified a 26 kDa band that corresponded to the NKX3.1 protein, while C-MYC showed a 50 kDa band on Western blotting analysis of all prostatic tissues. We observed that NKX3.1 protein and transcript were down-regulated in prostate cancer (PC) samples compared with non-neoplastic samples. We also observed that C-MYC protein was overexpressed in PC samples compared with normal (P=.001) and proliferative inflammatory atrophy (PIA) samples (P=.003). We found a positive correlation between NKX3.1 and C-MYC protein expression in normal and PIA samples. Interestingly, a negative correlation (NKX3.1 downregulation and MYC overexpression) was observed between NKX3.1 and MYC transcripts in PC. Thus, samples with higher C-MYC expression also exhibited higher NKX3.1 expression, which indicates the regulation of C-MYC by NKX3.1 protein. As in humans, these two genes and proteins were found to be related to canine prostate cancer. However, in contrast from what is observed in humans, in canine PC samples, the downregulation of NKX3.1 cannot be explained by DNA hypermethylation. (AU)

FAPESP's process: 10/13774-6 - Imunohistochemistry evaluation of c-Myc and NKX3.1 in tissue microarray (TMA) in pre-neoplastic and neoplastic lesions in canine prostate
Grantee:Carlos Eduardo Fonseca Alves
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/16068-0 - Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of c-myc, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs
Grantee:Renee Laufer Amorim
Support type: Regular Research Grants
FAPESP's process: 12/18426-1 - Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of c-Myc, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs
Grantee:Carlos Eduardo Fonseca Alves
Support type: Scholarships in Brazil - Doctorate