Advanced search
Start date
Betweenand

Impact of intrauterine fetal programming by low protein diet on rat prostate stem cells and morphogenesis

Abstract

Intrauterine fetal programming by low protein diet (IFP) impairs growth and maturation of rat prostate. Moreover, this kind of fetal programming increases the incidence of proliferative lesions in adult rat prostates, mainly lesions containing stem cells markers. Recent studies emphasize the role of stem cells in the initiation and progression of prostate cancer. Thus, the aim of this project is to investigate the impact of fetal programming by low protein diet on genes involved in prostatic morphogenesis and on prosatic stem cells proliferation/diferenciation. In this sense, the male offspring that born from rats fed a standard diet (group NP) or low protein diet (RP group) will be sacrificed at 3, 5, 10, 21 and 35 days of age. The ventral prostatic lobes (VP) will be dissected; the epithelial bud and the stem cells niche will be analysed throught free-floating imunofluorescence. The gene expression involved in prostatic morphogenesis and stem cells proliferation e/or differentiation (Sca-1, ABCG2, Nanog, CD133, CD117, Nkx3.1, BMP4, BMP7, FGF10, Hoxa13, Wnt2, Wnt5a, IGF-1R e Notch 1) will be investigated by qRT-PCR and western blotting. Moreover, the VP from NP and RP animals at day 10 postnatal will be cultured ex vivo for 11 days under exposure of diferente hormones (testosterone, estradiol and IGF-1) to analyse the stem cell proliferation and ductal morphogenesis. These results may help to elucidate some mechanisms by which IFP affects the prostate stem cells population and morphogenesis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMARGO, ANA C. L.; CONSTANTINO, FLAVIA B.; SANTOS, SERGIO A. A.; COLOMBELLI, KETLIN T.; DAL-PAI-SILVA, MAELI; FELISBINO, SERGIO L.; JUSTULIN, LUIS A.. Influence of postnatal prolactin modulation on the development and maturation of ventral prostate in young rats. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 30, n. 7, p. 969-979, . (13/24230-5)
CONSTANTINO, FLAVIA B.; CAMARGO, ANA C. L.; SANTOS, SERGIO A. A.; COLOMBELLI, KETLIN T.; MARTIN, LAURA F.; SILVA, MARCIA G.; FELISBINO, SERGIO L.; JUSTULIN, LUIS A.. The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement. Journal of Molecular Histology, v. 48, n. 5-6, p. 403-415, . (13/24230-5)
COLOMBELLI, KETLIN T.; SANTOS, SERGIO A. A.; CAMARGO, ANA C. L.; CONSTANTINO, FLAVIA B.; BARQUILHA, CAROLINE N.; RINALDI, JAQUELINE C.; FELISBINO, SERGIO L.; JUSTULIN, LUIS A.. Impairment of microvascular angiogenesis is associated with delay in prostatic development in rat offspring of maternal protein malnutrition. General and Comparative Endocrinology, v. 246, p. 258-269, . (13/24230-5)
SANTOS, SERGIO A. A.; CAMARGO, ANA C.; CONSTANTINO, FLAVIA B.; COLOMBELLI, KETLIN T.; MANI, FERNANDA; RINALDI, JAQUELINE C.; FRANCO, SUELEN; PORTELA, LUIZ M. F.; DURAN, BRUNO O. S.; SCARANO, WELLERSON R.; et al. Maternal Low-Protein Diet Impairs Prostate Growth in Young Rat Offspring and Induces Prostate Carcinogenesis With Aging. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, v. 74, n. 6, p. 751-759, . (13/24230-5, 14/08531-8, 17/01063-7)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.